Lactobacillus plantarum Disrupts S. mutans-C. albicans Cross-Kingdom Biofilms

Dental caries, an ecological dysbiosis of oral microflora, initiates from the virulent biofilms formed on tooth surfaces where cariogenic microorganisms metabolize dietary carbohydrates, producing acid that demineralizes tooth enamel. Forming cariogenic biofilms, and are well-recognized and emerging...

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Published in:Frontiers in cellular and infection microbiology 2022-03, Vol.12, p.872012-872012
Main Authors: Zeng, Yan, Fadaak, Ahmed, Alomeir, Nora, Wu, Tong Tong, Rustchenko, Elena, Qing, Shuang, Bao, Jianhang, Gilbert, Christie, Xiao, Jin
Format: Article
Language:English
Subjects:
Biofilms
Candida albicans
Candida albicans - physiology
Cellular and Infection Microbiology
cross-kingdom interactions
Dental Caries
Lactobacillus plantarum
multispecies biofilms
Streptococcus mutans
Streptococcus mutans - genetics
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Summary:Dental caries, an ecological dysbiosis of oral microflora, initiates from the virulent biofilms formed on tooth surfaces where cariogenic microorganisms metabolize dietary carbohydrates, producing acid that demineralizes tooth enamel. Forming cariogenic biofilms, and are well-recognized and emerging pathogens for dental caries. Recently, probiotics have demonstrated their potential in treating biofilm-related diseases, including caries. However, limited studies have assessed their effect on cariogenic bacteria-fungi cross-kingdom biofilm formation and their underlying interactions. Here, we assessed the effect of four probiotic ATCC 2836, ATCC 8014, ATCC 14917, and ATCC 11741) on and using a comprehensive multispecies biofilm model that mimicked high caries risk clinical conditions. Among the tested probiotic species, demonstrated superior inhibition on the growth of and , disruption of virulent biofilm formation with reduced bacteria and exopolysaccharide (EPS) components, and formation of virulent microcolonies structures. Transcriptome analysis (RNA sequencing) further revealed disruption of and cross-kingdom interactions with added . Genes of and involved in metabolic pathways (e.g., EPS formation, carbohydrate metabolism, glycan biosynthesis, and metabolism) were significantly downregulated. More significantly, genes related to resistance to antifungal medication (ERG4), fungal cell wall chitin remodeling (CHT2), and resistance to oxidative stress (CAT1) were also significantly downregulated. In contrast, genes , , and that contribute to antimicrobial peptide plantaricin production were significantly upregulated. Our novel study findings support further assessment of the potential role of probiotic for cariogenic biofilm control.
ISSN:2235-2988
2235-2988
DOI:10.3389/fcimb.2022.872012