Lipidomics identifies a requirement for peroxisomal function during influenza virus replication[S]

Influenza virus acquires a host-derived lipid envelope during budding, yet a convergent view on the role of host lipid metabolism during infection is lacking. Using a mass spectrometry-based lipidomics approach, we provide a systems-scale perspective on membrane lipid dynamics of infected human lung...

Full description

Saved in:
Bibliographic Details
Published in:Journal of lipid research 2014-07, Vol.55 (7), p.1357-1365
Main Authors: Tanner, Lukas Bahati, Chng, Charmaine, Guan, Xue Li, Lei, Zhengdeng, Rozen, Steven G., Wenk, Markus R.
Format: Article
Language:English
Subjects:
A549 Cells
Animals
biochemistry
CHO Cells
Cricetinae
Cricetulus
Dogs
ether lipids
glycerophospholipids
Humans
Influenza A Virus, H1N1 Subtype - physiology
lipid metabolism
Madin Darby Canine Kidney Cells
Peroxisomes - metabolism
Peroxisomes - virology
Phosphatidylcholines - metabolism
sphingolipids
systems biology
Virus Replication - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Influenza virus acquires a host-derived lipid envelope during budding, yet a convergent view on the role of host lipid metabolism during infection is lacking. Using a mass spectrometry-based lipidomics approach, we provide a systems-scale perspective on membrane lipid dynamics of infected human lung epithelial cells and purified influenza virions. We reveal enrichment of the minor peroxisome-derived ether-linked phosphatidylcholines relative to bulk ester-linked phosphatidylcholines in virions as a unique pathogenicity-dependent signature for influenza not found in other enveloped viruses. Strikingly, pharmacological and genetic interference with peroxisomal and ether lipid metabolism impaired influenza virus production. Further integration of our lipidomics results with published genomics and proteomics data corroborated altered peroxisomal lipid metabolism as a hallmark of influenza virus infection in vitro and in vivo. Influenza virus may therefore tailor peroxisomal and particularly ether lipid metabolism for efficient replication.
ISSN:0022-2275
1539-7262
DOI:10.1194/jlr.M049148