Fine mapping of Ascaris lumbricoides, Trichuris trichiura and hookworm infections in sub-districts of Makenene in Centre Region of Cameroun

• Published in: Scientific reports 2022-08, Vol.12 (1), p.13935-13935, Article 13935
• Main Authors: Kamdem, Cyrille Nguemnang, Tiofack, Auvaker Arnol Zebaze, Mewamba, Estelle Mezajou, Tchounkeu, Esthelline Yangea, Tatang, Joël Rostand Atiokeng, Mengoue, Edmond Loic Tekeu, Mbagnia, Carole Mureille Tchami, Fogue, Pythagore Soubgwi, Womeni, Hilaire Macaire, Simo, Gustave
• Format: Article
• Language: English
• Subjects: 692/308/174; 692/308/575; Ascaris lumbricoides; Chemotherapy; Disease hot spots; Geographic information systems; Humanities and Social Sciences; Infections; Mapping; multidisciplinary; Parasites; Public health; Remote sensing; Science; Science (multidisciplinary); Soils; Trichuris trichiura
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-18285-7

Preventive chemotherapy (PC) that remains the main control strategy recommended by the World Health Organization to achieve the elimination of soil-transmitted helminth (STH) infections as a public health problem must be strengthened by identifying the remaining transmission hot-spots for the deployment of appropriate control measures. This study was designed to assess the prevalence and infections intensities of soil-transmitted helminths and perform micro scale mapping in order to identify transmission hot-spots for targeted control operations. Stool samples were collected from 1775 children in ten primary schools of eight sub-districts of Makenene in Cameroon. Kato Katz technique was used to process and examine stool samples to detect the eggs of soil-transmitted nematodes. The prevalence of soil-transmitted helminth species as well as the infection intensities was compared. Data visualizations in forms of maps were made using Quantum geographic information system (QGIS) software. The overall prevalence of soil-transmitted helminth infections was 4.8% with a 95% confidence interval (CI) of 3.8–5.9%: 3.0% (95% CI 2.2–3.9) for A scaris lumbricoides , 1.4% (95% CI 0.9–2.0) for Trichuris trichiura and 0.8% (95% CI 0.5–1.4) for hookworms. The prevalence of soil-transmitted helminth species differ significantly between schools and sub-districts. The intensity of infections was light (2.4%, 1.1% and 0.8%), moderate (0.4%, 0.1% and 0.1%) and heavy (0.2%, 0.2% and 0%) for A. lumbricoides , T. trichiura and hookworm respectively. The mean intensity of infections was 7255 EPG for A. lumbricoides , 2900 EPG for T. trichiura and 298 EPG for hookworm. Between schools, significant difference was recorded in the means of infection intensities of T. Trichiura and hookworms but not for A. lumbricoides . This difference was also significant for T. Trichiura when comparison were between sex. No significant difference were recorded when the comparison were between age . Fine mapping revealed that children harbouring heavy infections were clustered in the same sub-districts; highlighting the presence of high endemicity sub-districts and hot-spots for the transmission of different soil-transmitted helminth species. This study showed a diversity in the prevalence and transmission of different soil-transmitted helminth species. It also hightlighted the need for micro scale mapping to enable the localisation of high endemicity sub-districts and transmission hot-spot sites where