Comprehensive genetic diversity and molecular evolutionary analysis of Theileria annulata isolates based on TAMS 1 gene

• Published in: Ticks and tick-borne diseases 2023-11, Vol.14 (6), p.102241-102241, Article 102241
• Main Authors: Paliwal, Sanjhi, Shanker, Daya, Sudan, Vikrant, Singh, Shanker Kumar
• Format: Article
• Language: English
• Subjects: Genetic diversity; Phylogenetic characterization; T. annulata; TAMS
• ISSN: 1877-959X; 1877-9603
• DOI: 10.1016/j.ttbdis.2023.102241

•Comprehensive evolutionary analysis of 337 T. annulata isolates from 16 countries based on TAMS 1 gene.•Complex star network of 92 Haplotypes, Hap_14 extensively circulating in 10 countries.•Negligible genetic distance (FST˂0.15) and very high gene flow (Nm˃1) among 5 populations support no geographical grouping in phylogenetic analysis.•Merozoite surface protein analysis placed T. annulata adjacent to T. lestoqaurdi. Molecular epidemiological studies related to the phylogenetic characterization of Theileria annulata are important in delineating the evolutionary history of the parasite. In the current study, the Theileria annulata (T. annulata) merozoite surface antigen 1 (TAMS 1) gene from 14 bovine isolates of T. annulata originating from semi-arid zone of northern India were amplified and sequenced. TAMS 1 gene sequences (n= 337) reported from 16 countries were subsequently analyzed for haplotype network along with genetic diversity. A total of five haplotypes out of the 14 sequenced isolates and 92 haplotypes out of 337 worldwide sequences are documented in this study. Phylogenetic and molecular evolutionary analyses based on TAMS 1 gene sequences showed that T. annulata is dissipated across different countries and numerous strains are closely linked, even though they belong to different geographical locations. The nucleotide homology between 14 isolates from northern India varied between 91.3 and 100%, whereas it was between 31.5 and 100% when sequences across the globe were compared. Haplotype 14 was recognized as most widely distributed haplotype, with 46 isolates circulating in 10 countries. Globally, negligible genetic distance (FST˂0.15) and very high gene flow (Nm˃1) was found in the five populations of the world (South Asia, East Asia, West Asia, Europe and Africa), supporting the absence of clearly defined subgroups in the phylogenetic analysis. Significant negative values of neutrality tests; Tajima's D (D) and Fu and Li's F (F) provided evidence for recent population expansion through positive selection of advantageous variations. [Display omitted]