Systematic Analysis of BELL Family Genes in Zizania latifolia and Functional Identification of ZlqSH1a/b in Rice Seed Shattering

The loss of seed shattering is an important event in crop domestication, and elucidating the genetic mechanisms underlying seed shattering can help reduce yield loss during crop production. This study is the first to systematically identify and analyse the BELL family of transcription factor-encodin...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-12, Vol.23 (24), p.15939
Main Authors: Xie, Yan-Ning, Yang, Ting, Zhang, Bin-Tao, Qi, Qian-Qian, Ding, An-Ming, Shang, Lian-Guang, Zhang, Yu, Qian, Qian, Zhang, Zhong-Feng, Yan, Ning
Format: Article
Language:English
Subjects:
Abscission
abscission layer
Agricultural production
Amino acids
Chinese wild rice (Zizania latifolia)
Cloning
Crop production
Cultivars
Domestication
Edible Grain - genetics
Genes
Genes, Plant
Genomes
Grain
Oryza
Overexpression
Proteins
Rice
rice (Oryza sativa)
seed shattering
Seeds
Transcriptomes
Zizania latifolia
ZlBELL transcription factor
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Summary:The loss of seed shattering is an important event in crop domestication, and elucidating the genetic mechanisms underlying seed shattering can help reduce yield loss during crop production. This study is the first to systematically identify and analyse the BELL family of transcription factor-encoding genes in Chinese wild rice ( ). (Zla04G033720) and (Zla02G027130) were identified as key candidate genes involved in seed shattering in . These genes were involved in regulating the development of the abscission layer (AL) and were located in the nucleus of the cell. Over-expression of and resulted in a complete AL between the grain and pedicel and significantly enhanced seed shattering after grain maturation in rice. Transcriptome sequencing revealed that 172 genes were differentially expressed between the wild type (WT) and the two transgenic ( and over-expressing) plants. Three of the differentially expressed genes related to seed shattering were validated using qRT-PCR analysis. These results indicate that and are involved in AL development in rice grains, thereby regulating seed shattering. Our results could facilitate the genetic improvement of seed-shattering behaviour in and other cereal crops.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms232415939