Identification and fine mapping of Bph33, a new brown planthopper resistance gene in rice (Oryza sativa L.)

• Published in: Rice (New York, N.Y.) N.Y.), 2018-10, Vol.11 (1), p.55-12, Article 55
• Main Authors: Hu, Jie, Chang, Xingyuan, Zou, Ling, Tang, Weiqi, Wu, Weiren
• Format: Article
• Language: English
• Subjects: Agriculture; Antibiosis; Biomedical and Life Sciences; Biotypes; Chromosome 4; Cloning; Cultivars; Gene expression; Gene mapping; Genes; Host plants; Leucine; Life Sciences; Marker-assisted selection; Original; Original Article; Oryza sativa; Plant Breeding/Biotechnology; Plant Ecology; Plant Genetics and Genomics; Plant resistance; Plant Sciences; Population studies; Quantitative trait loci; Seedlings
• ISSN: 1939-8425; 1939-8433; 1934-8037
• DOI: 10.1186/s12284-018-0249-7

Background Host-plant resistance is the most desirable and economic way to overcome BPH damage to rice. As single-gene resistance is easily lost due to the evolution of new BPH biotypes, it is urgent to explore and identify new BPH resistance genes. Results In this study, using F 2:3 populations and near-isogenic lines (NILs) derived from crosses between two BPH-resistant Sri Lankan rice cultivars (KOLAYAL and POLIYAL) and a BPH-susceptible cultivar 9311, a new resistance gene Bph33 was fine mapped to a 60-kb region ranging 0.91–0.97 Mb on the short arm of chromosome 4 (4S), which was at least 4 Mb distant from those genes/QTLs ( Bph12 , Bph15 , Bph3 , Bph20 , QBph4 and QBph4.2 ) reported before. Seven genes were predicted in this region. Based on sequence and expression analyses, a Leucine Rich Repeat (LRR) family gene (LOC_Os04g02520) was identified as the most possible candidate of Bph33 . The gene exhibited continuous and stable resistance from seedling stage to tillering stage, showing both antixenosis and antibiosis effects on BPH. Conclusion The results of this study will facilitate map-based cloning and marker-assisted selection of the gene.