Proteomic Characterization of the Oral Pathogen Filifactor alocis Reveals Key Inter-Protein Interactions of Its RTX Toxin: FtxA

is a Gram-positive asaccharolytic, obligate anaerobic rod that has been isolated from a variety of oral infections including periodontitis, peri-implantitis, and odontogenic abscesses. As a newly emerging pathogen, its type strain has been investigated for pathogenic properties, yet little is known...

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Bibliographic Details
Published in:Pathogens (Basel) 2022-05, Vol.11 (5), p.590
Main Authors: Bao, Kai, Claesson, Rolf, Gehrig, Peter, Grossmann, Jonas, Oscarsson, Jan, Belibasakis, Georgios N
Format: Article
Language:English
Subjects:
ABC transporters
Abscesses
Antibiotics
Catalytic activity
Dehydrogenases
DNA repair
Drug resistance
Filifactor alocis
FtxA
Genes
Genomes
label-free quantification proteomics
Medicin och hälsovetenskap
Pathogens
Periodontitis
Phylogenetics
Protein interaction
Proteins
Proteomes
Proteomics
Toxins
Virulence
Virulence factors
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Summary:is a Gram-positive asaccharolytic, obligate anaerobic rod that has been isolated from a variety of oral infections including periodontitis, peri-implantitis, and odontogenic abscesses. As a newly emerging pathogen, its type strain has been investigated for pathogenic properties, yet little is known about its virulence variations among strains. We previously screened the whole genome of nine clinical oral isolates and a reference strain of , and they expressed a novel RTX toxin, FtxA. In the present study, we aimed to use label-free quantification proteomics to characterize the full proteome of those ten strains. A total of 872 proteins were quantified, and 97 among them were differentially expressed in FtxA-positive strains compared with the negative strains. In addition, 44 of these differentially expressed proteins formed 66 pairs of associations based on their predicted functions, which included clusters of proteins with DNA repair/mediated transformation and catalytic activity-related function, indicating different biosynthetic activities among strains. FtxA displayed specific interactions with another six intracellular proteins, forming a functional cluster that could discriminate between FtxA-producing and non-producing strains. Among them were FtxB and FtxD, predicted to be encoded by the same operon as FtxA. While revealing the broader qualitative and quantitative proteomic landscape of , this study also sheds light on the deeper functional inter-relationships of FtxA, thus placing this RTX family member into context as a major virulence factor of this species.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens11050590