Spatially Resolved Transcriptomics Technology Facilitates Cancer Research

Single cell RNA sequencing (scRNA-seq) provides a great convenience for studying tumor occurrence and development for its ability to study gene expression at the individual cell level. However, patient-derived tumor tissues are composed of multiple types of cells including tumor cells and adjacent n...

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Bibliographic Details
Published in:Advanced science 2023-10, Vol.10 (30), p.e2302558-n/a
Main Authors: Wang, Qian, Zhi, Yuan, Zi, Moxin, Mo, Yongzhen, Wang, Yumin, Liao, Qianjin, Zhang, Shanshan, Gong, Zhaojian, Wang, Fuyan, Zeng, Zhaoyang, Guo, Can, Xiong, Wei
Format: Article
Language:English
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Cancer research
cancer‐associated fibroblast (CAF)
Gene expression
Gene Expression Profiling
High-Throughput Nucleotide Sequencing
Humans
Hybridization
Immunotherapy
Medical research
Neoplasms - genetics
Review
Reviews
Signal to noise ratio
spatially resolved transcriptomics (SRT)
Technology
tertiary lymphoid structure (TLS)
Transcriptome - genetics
tumor heterogeneity
tumor immune microenvironment
Tumors
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Summary:Single cell RNA sequencing (scRNA-seq) provides a great convenience for studying tumor occurrence and development for its ability to study gene expression at the individual cell level. However, patient-derived tumor tissues are composed of multiple types of cells including tumor cells and adjacent non-malignant cells such as stromal cells and immune cells. The spatial locations of various cells in situ tissues plays a pivotal role in the occurrence and development of tumors, which cannot be elucidated by scRNA-seq alone. Spatially resolved transcriptomics (SRT) technology emerges timely to explore the unrecognized relationship between the spatial background of a particular cell and its functions, and is increasingly used in cancer research. This review provides a systematic overview of the SRT technologies that are developed, in particular the more widely used cutting-edge SRT technologies based on next-generation sequencing (NGS). In addition, the main achievements by SRT technologies in precisely unveiling the underappreciated spatial locations on gene expression and cell function with unprecedented high-resolution in cancer research are emphasized, with the aim of developing more effective clinical therapeutics oriented to a deeper understanding of the interaction between tumor cells and surrounding non-malignant cells.
ISSN:2198-3844
2198-3844
DOI:10.1002/advs.202302558