Detailed structural analyses and viscoelastic properties of nano-fibrillated bacterial celluloses

• Published in: Carbohydrate polymer technologies and applications 2024-12, Vol.8, p.100565, Article 100565
• Main Authors: Tsujisaki, Haruto, Hosokawa, Masaaki, Takasaki, Yuichi, Yamagata, Yoshifumi, Kawabata, Yui, Tatsumi, Daisuke, Seno, Shuichiro, Miyamoto, Keisuke, Isono, Takuya, Yamamoto, Takuya, Tani, Hirofumi, Satoh, Toshifumi, Orihara, Hiroshi, Tajima, Kenji
• Format: Article
• Language: English
• Subjects: Bacterial cellulose; Large aspect ratio; Long fiber length; Nanocellulose; Viscoelastic properties
• ISSN: 2666-8939; 2666-8939
• DOI: 10.1016/j.carpta.2024.100565

•Fiber lengths of NFBCs are about 17 μm and aspect ratios are >1000.•NFBCs have fiber-to-fiber interactions even at low concentrations (0.01 wt%).•NFBCs maintain stable gel-like properties across 0.2–1.0 wt%.•NFBCs can modify material properties even at low concentrations. Nano-fibrillated bacterial cellulose (NFBC) can be prepared by cultivating a cellulose-producing bacterium in a medium containing a dispersant under agitating and aerobic conditions. Although NFBCs have various applications, their detailed structure and physical properties have not been clarified. Therefore, in this study, we performed detailed structural and physical property analyses of NFBCs to advance their potential applications. Atomic force microscopy and image analysis showed that the average fiber length of NFBCs was approximately 17 µm and fiber widths were 10–15 nm; the aspect ratios of NFBCs were > 1000, which are >10-fold higher than that of 2,2,6,6-tetramethylpioeridine-1-oxyl-oxidized cellulose nanofiber. Shear viscosity measurements showed that the NFBCs exhibited shear-thinning flow behavior even at low concentrations (0.01 wt%). Frequency sweep measurements showed that the storage modulus values were greater than the loss modulus values in the measured frequency range, indicating that the NFBCs were in a stable gel state. Thus, the NFBCs exhibited significantly longer fiber lengths, larger aspect ratios, and excellent viscoelastic properties based on these unique structural features. Our findings will help develop novel applications utilizing the ultrahigh aspect ratio unique to NFBC and its viscoelastic properties. [Display omitted]