Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules

• Published in: Nature communications 2022-09, Vol.13 (1), p.5584-5584, Article 5584
• Main Authors: Wang, Tao, Tian, Xibin, Kim, Han Byeol, Jang, Yura, Huang, Zhiyuan, Na, Chan Hyun, Wang, Jiou
• Format: Article
• Language: English
• Subjects: 14/1; 14/19; 631/337; 631/378/1689/1285; 631/80/304; 631/80/86/2366; Amyotrophic lateral sclerosis; Dementia disorders; Energy; Energy metabolism; Frontotemporal dementia; Granular materials; Humanities and Social Sciences; Initiation factor eIF-4E; Intracellular; multidisciplinary; Neurodegeneration; Neurodegenerative diseases; Organelles; Organoids; Pathogenesis; Phosphorylation; Science; Science (multidisciplinary); TOR protein
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-022-33079-1

Energy metabolism and membraneless organelles have been implicated in human diseases including neurodegeneration. How energy deficiency regulates ribonucleoprotein particles such as stress granules (SGs) is still unclear. Here we identified a unique type of granules induced by energy deficiency under physiological conditions and uncovered the mechanisms by which the dynamics of diverse stress-induced granules are regulated. Severe energy deficiency induced the rapid formation of energy deficiency-induced stress granules (eSGs) independently of eIF2α phosphorylation, whereas moderate energy deficiency delayed the clearance of conventional SGs. The formation of eSGs or the clearance of SGs was regulated by the mTOR-4EBP1-eIF4E pathway or eIF4A1, involving assembly of the eIF4F complex or RNA condensation, respectively. In neurons or brain organoids derived from patients carrying the C9orf72 repeat expansion associated with amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), the eSG formation was enhanced, and the clearance of conventional SGs was impaired. These results reveal a critical role for intracellular energy in the regulation of diverse granules and suggest that disruptions in energy-controlled granule dynamics may contribute to the pathogenesis of relevant diseases. Stress granules are associated with neurodegenerative diseases. Here, Wang et al. found intracellular energy deficiencies trigger a unique type of granules and disrupt granule disassembly through 4EBP1/eIF4A.