Role of human dural fibroblasts in the angiogenic responses of human endothelial cells: An in vitro dural model and the application of lab-on-a-chip for EDAS

Encephaloduroarteriosynangiosis (EDAS), an indirect anastomosis procedure, is widely accepted as a primary treatment for moyamoya disease (MMD) to improve collateral blood flow. During surgical intervention, dural fibroblasts (DuF) are thought to produce various proteins that create an angiogenic mi...

Full description

Saved in:
Bibliographic Details
Published in:Bioengineering & translational medicine 2023-11, Vol.8 (6), p.e10589-n/a
Main Authors: Kwon, Woo-Keun, Yoo, Chang-Min, Kim, Jang Hun, Kim, Tae-Won, Kim, An-Gi, Hwang, Min-Ho, Choi, Hyuk
Format: Article
Language:English
Subjects:
Angiogenesis
Biomarkers
Blood flow
Chemokines
Cytokines
Disease
dura mater
Encephaloduroarteriosynangiosis
Endothelial cells
Fibroblasts
Genes
inflammation
Lab-on-a-chip
Microfluidics
Moyamoya disease
Neutrophils
Proteins
Transcription factors
Veins & arteries
Wound healing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Encephaloduroarteriosynangiosis (EDAS), an indirect anastomosis procedure, is widely accepted as a primary treatment for moyamoya disease (MMD) to improve collateral blood flow. During surgical intervention, dural fibroblasts (DuF) are thought to produce various proteins that create an angiogenic microenvironment. However, the biophysiological evidence supporting the angiogenic properties of this surgical technique has not been thoroughly elucidated. The purpose of these studies was to determine whether DuF releases pro-angiogenic factors and chemokines and promotes angiogenic properties in human endothelial cells (ECs) under IL-1β-mediated wound conditions, which are expected to occur during the process of neo-vascularization within the dura mater. Furthermore, a microfluidic chemotaxis platform was implemented to investigate the angiogenic activity of ECs in response to a reconstituted dura model. Transcriptome sequencing revealed that IL-1β stimulation on DuF induced a significant upregulation of various pro-angiogenic genes, including IL-6, IL-8, CCL-2, CCL-5, SMOC-1, and SCG-2 (  
ISSN:2380-6761
2380-6761
DOI:10.1002/btm2.10589