Mycoremediation of lead and cadmium by lignocellulosic enzymes of Pleurotus eryngii

• Published in: AMB Express 2023-11, Vol.13 (1), p.127-127, Article 127
• Main Authors: Goligar, N., Saadatmand, S., Khavarinejad, R. A.
• Format: Article
• Language: English
• Subjects: Atomic absorption spectroscopy; Biomedical and Life Sciences; Biotechnology; Cadmium; Culture media; Enzymes; Flame photometry; Fungi; Gene banks; Heavy metals; Industrial wastes; Lead; Life Sciences; Lignocellulose; Microbial Genetics and Genomics; Microbiology; Mycoremediation; Original Article; Oyster mushrooms; Photometry; Pleurotus eryngii; Sanger Sequencing
• ISSN: 2191-0855; 2191-0855
• DOI: 10.1186/s13568-023-01626-8

This study aimed to investigate the ability of Pleurotus eryngii fungus to absorb lead and cadmium from industrial wastewater. After culturing the fungus on a potato dextrose agar (PDA) medium containing 0 (control), 150 mg L −1 , 250 mg L −1 , and 350 mg L −1 concentrations of lead and cademium for 30 days, the mycelia were isolated from the culture medium and their extracts were used to measure protein content and the activity of antioxidant enzymes. Also, heavy metal contents were analyzed by atomic absorption spectrometry using flame photometry. Results showed that the growth of mycelia was significantly affected by different concentrations of the two heavy metals. High tolerance of heavy metal pollution in the culture media and the ability to accumulate lead and cademium confirmed that Pleurotus eryngii is a favorable option for mycoremediation. Also, molecular studies for fungal sequencing were investigated using the trench method, the sequence of the fungus was recorded in the gene bank, and finally the fungus was identified in the study. Key points Mycoremediation is a fast and low-cost heavy metal removal solutiok The proposed process does not add any harmful substances to the environment after cleaning.