Characterization of Some Salt-Tolerant Bacterial Hydrolases with Potential Utility in Cultural Heritage Bio-Cleaning

Salt-tolerant enzymes produced by halophilic and halotolerant microorganisms have been proposed to be used in various applications that involve high saline conditions. Considering their biotechnological significance and the current need for more efficient producers of such catalysts, the present stu...

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Bibliographic Details
Published in:Microorganisms (Basel) 2022-03, Vol.10 (3), p.644
Main Authors: Ruginescu, Robert, Enache, Madalin, Popescu, Octavian, Gomoiu, Ioana, Cojoc, Roxana, Batrinescu-Moteau, Costin, Maria, Gabriel, Dumbravician, Maria, Neagu, Simona
Format: Article
Language:English
Subjects:
Bacillus
Bacteria
Biocatalysts
Biotechnology
Catalysts
Cleaning
Cultural heritage
Cultural resources
Culture
Enzymes
esterase
Esterases
Fungi
Glucose
halophilic
halotolerant
hydrolase
Laboratories
Microorganisms
pH effects
Proteolysis
Salinity tolerance
Salt
Salts
Sodium chloride
Strains (organisms)
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Summary:Salt-tolerant enzymes produced by halophilic and halotolerant microorganisms have been proposed to be used in various applications that involve high saline conditions. Considering their biotechnological significance and the current need for more efficient producers of such catalysts, the present study aimed to evaluate the extracellular proteolytic, esterolytic, cellulolytic and xylanolytic activities of some halotolerant strains, and to characterize their functional parameters. A total of 21 bacterial and fungal strains belonging to the genera , , , , , , , , and were assayed by quantitative methods. Among them, the members of the genus exhibited the highest catalytic activities. The exoenzymes produced by three selected strains were active over wide ranges of salinity, temperature and pH. Proteases were active at 20-80 °C, pH 6-10, and 0-1 M NaCl, while esterases showed good catalytic activities at 20-80 °C, pH 7.5-10, and 0-4 M NaCl. Cellulases and xylanases were active at 20-80 °C, pH 5-10, and 0-5 M NaCl. Due to such properties, these hydrolases could be used in a newly proposed application, namely to clean aged consolidants and organic deposits accumulated over time from the surfaces of salt-loaded wall paintings.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms10030644