Bisphenol F suppresses insulin-stimulated glucose metabolism in adipocytes by inhibiting IRS-1/PI3K/AKT pathway

• Published in: Ecotoxicology and environmental safety 2022-02, Vol.231, p.113201-113201, Article 113201
• Main Authors: Chen, Huiling, Li, Jiangbin, Zhang, Yanchao, Zhang, Wei, Li, Xing, Tang, Huanwen, Liu, Yungang, Li, Tianlan, He, Haoqi, Du, Bohai, Li, Li, Shi, Ming
• Format: Article
• Language: English
• Subjects: Adipocyte; Adipocytes; Adipokine; Animals; Benzhydryl Compounds; Bisphenol F; Glucose; Glucose metabolism; Insulin; IRS-1/PI3K/Akt pathway; Phenols; Phosphatidylinositol 3-Kinases - genetics; Proto-Oncogene Proteins c-akt - genetics
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2022.113201

Obesity is one of the risk factors of metabolic diseases. Decreased sensitivity to insulin or impairment of the insulin signaling pathway may affect the metabolism of adipose tissue. Bisphenol F (BPF) has been widely used in various products as a substitute for bisphenol A (BPA). BPA has been defined as “obesogen”. However, knowledge about the correlation between BPF and obesity is very limited. This study was aimed to explore the effects of BPF on glucose metabolism and insulin sensitivity in mammalian tissues, using a mouse 3T3-L1 adipocyte line as the model. Differentiated 3T3-L1 adipocytes were treated with BPF at various concentrations for 24 h or 48 h, followed by the measurement of cell viability, lipid accumulation, expression levels of adipocytokines, glucose consumption, and impairment of the insulin signaling pathway. The results indicated that BPF had no effect on the size of 3T3-L1 adipocytes, but the expression of leptin, adiponectin and apelin was decreased, while that of chemerin and resistin was increased after 48 h of BPF treatment. Moreover, BPF inhibited the glucose consumption, the expression of GLUT4, and its translocation to the plasma membranes in 3T3-L1 adipocytes. Western blot analysis indicated that the activation of IRS-1/PI3K/AKT signaling pathway was inhibited by BPF, which resulted in reduced GLUT4 translocation. In conclusion, our data suggest that exposure of adipocytes to BPF may alter the expression of calorie metabolism-related adipokines and suppress insulin-stimulated glucose metabolism by impairing the insulin signaling (IRS-1/PI3K/AKT) pathway. •Bisphenol F had no effect on the lipid accumulation of 3T3-L1 adipocytes.•Bisphenol F influenced the expression of adipokines in 3T3-L1 adipocytes.•Bisphenol F could inhibit glucose metabolism in 3T3-L1 adipocytes.•Bisphenol F could inhibit the glucose metabolism via glucose transporter type 4.•Bisphenol F could inhibit the IRS-1/PI3K/AKT pathway in insulin-treated adipocytes.