Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregation

Mesenchymal condensation is a critical step in organogenesis, yet the underlying molecular and cellular mechanisms remain poorly understood. The hair follicle dermal condensate is the precursor to the permanent mesenchymal unit of the hair follicle, the dermal papilla, which regulates hair cycling t...

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Bibliographic Details
Published in:eLife 2018-07, Vol.7
Main Authors: Biggs, Leah C, Mäkelä, Otto Jm, Myllymäki, Satu-Marja, Das Roy, Rishi, Närhi, Katja, Pispa, Johanna, Mustonen, Tuija, Mikkola, Marja L
Format: Article
Language:English
Subjects:
Actins - metabolism
Analysis
Animals
Cell Aggregation
Cell Biology
Cell Cycle
Cell Lineage
Cell Movement
Cell Shape
Criminal investigation
dermal condensate
Dermis - cytology
Dermis - ultrastructure
Developmental Biology
Embryo
Epithelium
Fgf20
Fibroblast Growth Factor 9 - pharmacology
Fibroblast growth factors
Fibroblast Growth Factors - metabolism
Fibroblasts - cytology
Fibroblasts - metabolism
Genes
hair follicle
Hair Follicle - cytology
live imaging
Mice, Inbred C57BL
Microscopy
Morphogenesis
Receptors, Fibroblast Growth Factor - metabolism
Receptors, Vascular Endothelial Growth Factor - metabolism
RNA
RNA sequencing
RNAseq
Signal Transduction
Skin
SOXB1 Transcription Factors - metabolism
Stem cells
Transcription, Genetic
Travel
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Summary:Mesenchymal condensation is a critical step in organogenesis, yet the underlying molecular and cellular mechanisms remain poorly understood. The hair follicle dermal condensate is the precursor to the permanent mesenchymal unit of the hair follicle, the dermal papilla, which regulates hair cycling throughout life and bears hair inductive potential. Dermal condensate morphogenesis depends on epithelial Fibroblast Growth Factor 20 (Fgf20). Here, we combine mouse models with 3D and 4D microscopy to demonstrate that dermal condensates form de novo and via directional migration. We identify cell cycle exit and cell shape changes as early hallmarks of dermal condensate morphogenesis and find that Fgf20 primes these cellular behaviors and enhances cell motility and condensation. RNAseq profiling of immediate Fgf20 targets revealed induction of a subset of dermal condensate marker genes. Collectively, these data indicate that dermal condensation occurs via directed cell movement and that Fgf20 orchestrates the early cellular and molecular events.
ISSN:2050-084X
2050-084X
DOI:10.7554/elife.36468