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Exploring MicroRNAs Associated with Pomegranate Pistil Development: An Identification and Analysis Study
The interaction between miRNAs (microRNAs) and target genes plays an important role in plant pistil development. MiRNAs related to pistils were explored in pomegranate. The differentially expressed miRNAs were screened at different developmental stages of pomegranate pistils, and their target differ...
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| Published in: | Horticulturae 2024-01, Vol.10 (1), p.85 |
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| container_start_page | 85 |
| container_title | Horticulturae |
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| creator | Zhao, Yujie Huang, Jingyi Li, Ming Ren, Hongfang Jiao, Jian Wan, Ran Liu, Yu Wang, Miaomiao Shi, Jiangli Zhang, Kunxi Hao, Pengbo Song, Shangwei Bai, Tuanhui Zheng, Xianbo |
| description | The interaction between miRNAs (microRNAs) and target genes plays an important role in plant pistil development. MiRNAs related to pistils were explored in pomegranate. The differentially expressed miRNAs were screened at different developmental stages of pomegranate pistils, and their target differentially expressed mRNAs were further identified to clarify the regulatory effect of miRNAs on pistil development. In our study, 61 conserved miRNAs were identified in 30 families, including miR395, miR394, miR393, miR161, miR162, and miR168. Among them, miR156, miR157, miR159, miR160, miR164, miR165, miR166, miR167, miR169, and miR172 were involved in the development of flower organs. Eight miRNAs were randomly selected and verified for qRT-PCR analysis. The result analysis indicated that miR160, miR164, and miR172 might be positive factors in the regulation of pomegranate pistil development. MiR156 and miR166 might be involved in regulation of pomegranate pistil development as negative factors. |
| doi_str_mv | 10.3390/horticulturae10010085 |
| format | article |
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MiRNAs related to pistils were explored in pomegranate. The differentially expressed miRNAs were screened at different developmental stages of pomegranate pistils, and their target differentially expressed mRNAs were further identified to clarify the regulatory effect of miRNAs on pistil development. In our study, 61 conserved miRNAs were identified in 30 families, including miR395, miR394, miR393, miR161, miR162, and miR168. Among them, miR156, miR157, miR159, miR160, miR164, miR165, miR166, miR167, miR169, and miR172 were involved in the development of flower organs. Eight miRNAs were randomly selected and verified for qRT-PCR analysis. The result analysis indicated that miR160, miR164, and miR172 might be positive factors in the regulation of pomegranate pistil development. MiR156 and miR166 might be involved in regulation of pomegranate pistil development as negative factors.</description><identifier>ISSN: 2311-7524</identifier><identifier>EISSN: 2311-7524</identifier><identifier>DOI: 10.3390/horticulturae10010085</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Binomial distribution ; Cell division ; correlation analysis ; Data analysis ; Developmental stages ; Females ; Flowers ; Genes ; Genetic aspects ; Genomes ; Identification and classification ; MicroRNA ; MicroRNAs ; miRNA ; Physiological aspects ; pistil ; Pistils ; Plant growth ; Pomegranate ; Proteins ; Regulation ; RNA polymerase ; Software</subject><ispartof>Horticulturae, 2024-01, Vol.10 (1), p.85</ispartof><rights>COPYRIGHT 2024 MDPI AG</rights><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c381t-e116120327b3d945821d3a2330e4cc06fb502b91ab8246654edf9fc4cb55a0863</cites><orcidid>0000-0002-1121-6742 ; 0000-0002-1816-9619 ; 0000-0001-5681-3981 ; 0000-0002-2117-3782 ; 0000-0002-9267-6600</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2918744955/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2918744955?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,44590,75126</link.rule.ids></links><search><creatorcontrib>Zhao, Yujie</creatorcontrib><creatorcontrib>Huang, Jingyi</creatorcontrib><creatorcontrib>Li, Ming</creatorcontrib><creatorcontrib>Ren, Hongfang</creatorcontrib><creatorcontrib>Jiao, Jian</creatorcontrib><creatorcontrib>Wan, Ran</creatorcontrib><creatorcontrib>Liu, Yu</creatorcontrib><creatorcontrib>Wang, Miaomiao</creatorcontrib><creatorcontrib>Shi, Jiangli</creatorcontrib><creatorcontrib>Zhang, Kunxi</creatorcontrib><creatorcontrib>Hao, Pengbo</creatorcontrib><creatorcontrib>Song, Shangwei</creatorcontrib><creatorcontrib>Bai, Tuanhui</creatorcontrib><creatorcontrib>Zheng, Xianbo</creatorcontrib><title>Exploring MicroRNAs Associated with Pomegranate Pistil Development: An Identification and Analysis Study</title><title>Horticulturae</title><description>The interaction between miRNAs (microRNAs) and target genes plays an important role in plant pistil development. MiRNAs related to pistils were explored in pomegranate. The differentially expressed miRNAs were screened at different developmental stages of pomegranate pistils, and their target differentially expressed mRNAs were further identified to clarify the regulatory effect of miRNAs on pistil development. In our study, 61 conserved miRNAs were identified in 30 families, including miR395, miR394, miR393, miR161, miR162, and miR168. Among them, miR156, miR157, miR159, miR160, miR164, miR165, miR166, miR167, miR169, and miR172 were involved in the development of flower organs. Eight miRNAs were randomly selected and verified for qRT-PCR analysis. The result analysis indicated that miR160, miR164, and miR172 might be positive factors in the regulation of pomegranate pistil development. MiR156 and miR166 might be involved in regulation of pomegranate pistil development as negative factors.</description><subject>Binomial distribution</subject><subject>Cell division</subject><subject>correlation analysis</subject><subject>Data analysis</subject><subject>Developmental stages</subject><subject>Females</subject><subject>Flowers</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Identification and classification</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>miRNA</subject><subject>Physiological aspects</subject><subject>pistil</subject><subject>Pistils</subject><subject>Plant growth</subject><subject>Pomegranate</subject><subject>Proteins</subject><subject>Regulation</subject><subject>RNA polymerase</subject><subject>Software</subject><issn>2311-7524</issn><issn>2311-7524</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkdtqGzEQhpfSQE2SRygIeu10dNrV5m5J0sbgNiFJr4VWB1tmvXIlbRq_feS4lF6EEczwM_Ohf6aqPmO4oLSFr-sQs9fTkKeoLAYoT_AP1YxQjOcNJ-zjf_Wn6jylDQAQYHXdkFm1vnnZDSH6cYV-eB3Dw88uoS6loL3K1qA_Pq_RfdjaVVRjUdC9T9kP6No-2yHstnbMl6gb0cKUyjuvVfZhRGo0RVXDPvmEHvNk9mfViVNDsud_82n169vN09XtfHn3fXHVLeeaCpznFuMaE6Ck6alpGRcEG6oIpWCZ1lC7ngPpW6x6QYoFzqxxrdNM95wrEDU9rRZHrglqI3fRb1Xcy6C8fBNCXEl12NhgZWNwXwPnNQbOmAGhqevbwjaYYcd5YX05snYx_J5synITplhsJUlaLBrG2reui2PXShWoH13IUekSxm69DqN1vuhdI0A0xRstA_w4UPadUrTu3zcxyMNR5btHpa8Fz5fj</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Zhao, Yujie</creator><creator>Huang, Jingyi</creator><creator>Li, Ming</creator><creator>Ren, Hongfang</creator><creator>Jiao, Jian</creator><creator>Wan, Ran</creator><creator>Liu, Yu</creator><creator>Wang, Miaomiao</creator><creator>Shi, Jiangli</creator><creator>Zhang, Kunxi</creator><creator>Hao, Pengbo</creator><creator>Song, Shangwei</creator><creator>Bai, Tuanhui</creator><creator>Zheng, Xianbo</creator><general>MDPI AG</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1121-6742</orcidid><orcidid>https://orcid.org/0000-0002-1816-9619</orcidid><orcidid>https://orcid.org/0000-0001-5681-3981</orcidid><orcidid>https://orcid.org/0000-0002-2117-3782</orcidid><orcidid>https://orcid.org/0000-0002-9267-6600</orcidid></search><sort><creationdate>20240101</creationdate><title>Exploring MicroRNAs Associated with Pomegranate Pistil Development: An Identification and Analysis Study</title><author>Zhao, Yujie ; Huang, Jingyi ; Li, Ming ; Ren, Hongfang ; Jiao, Jian ; Wan, Ran ; Liu, Yu ; Wang, Miaomiao ; Shi, Jiangli ; Zhang, Kunxi ; Hao, Pengbo ; Song, Shangwei ; Bai, Tuanhui ; Zheng, Xianbo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-e116120327b3d945821d3a2330e4cc06fb502b91ab8246654edf9fc4cb55a0863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Binomial distribution</topic><topic>Cell division</topic><topic>correlation analysis</topic><topic>Data analysis</topic><topic>Developmental stages</topic><topic>Females</topic><topic>Flowers</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Identification and classification</topic><topic>MicroRNA</topic><topic>MicroRNAs</topic><topic>miRNA</topic><topic>Physiological aspects</topic><topic>pistil</topic><topic>Pistils</topic><topic>Plant growth</topic><topic>Pomegranate</topic><topic>Proteins</topic><topic>Regulation</topic><topic>RNA polymerase</topic><topic>Software</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Yujie</creatorcontrib><creatorcontrib>Huang, Jingyi</creatorcontrib><creatorcontrib>Li, Ming</creatorcontrib><creatorcontrib>Ren, Hongfang</creatorcontrib><creatorcontrib>Jiao, Jian</creatorcontrib><creatorcontrib>Wan, Ran</creatorcontrib><creatorcontrib>Liu, Yu</creatorcontrib><creatorcontrib>Wang, Miaomiao</creatorcontrib><creatorcontrib>Shi, Jiangli</creatorcontrib><creatorcontrib>Zhang, Kunxi</creatorcontrib><creatorcontrib>Hao, Pengbo</creatorcontrib><creatorcontrib>Song, Shangwei</creatorcontrib><creatorcontrib>Bai, Tuanhui</creatorcontrib><creatorcontrib>Zheng, Xianbo</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>SciTech Premium Collection</collection><collection>Agriculture Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Horticulturae</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Yujie</au><au>Huang, Jingyi</au><au>Li, Ming</au><au>Ren, Hongfang</au><au>Jiao, Jian</au><au>Wan, Ran</au><au>Liu, Yu</au><au>Wang, Miaomiao</au><au>Shi, Jiangli</au><au>Zhang, Kunxi</au><au>Hao, Pengbo</au><au>Song, Shangwei</au><au>Bai, Tuanhui</au><au>Zheng, Xianbo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exploring MicroRNAs Associated with Pomegranate Pistil Development: An Identification and Analysis Study</atitle><jtitle>Horticulturae</jtitle><date>2024-01-01</date><risdate>2024</risdate><volume>10</volume><issue>1</issue><spage>85</spage><pages>85-</pages><issn>2311-7524</issn><eissn>2311-7524</eissn><abstract>The interaction between miRNAs (microRNAs) and target genes plays an important role in plant pistil development. MiRNAs related to pistils were explored in pomegranate. The differentially expressed miRNAs were screened at different developmental stages of pomegranate pistils, and their target differentially expressed mRNAs were further identified to clarify the regulatory effect of miRNAs on pistil development. In our study, 61 conserved miRNAs were identified in 30 families, including miR395, miR394, miR393, miR161, miR162, and miR168. Among them, miR156, miR157, miR159, miR160, miR164, miR165, miR166, miR167, miR169, and miR172 were involved in the development of flower organs. Eight miRNAs were randomly selected and verified for qRT-PCR analysis. The result analysis indicated that miR160, miR164, and miR172 might be positive factors in the regulation of pomegranate pistil development. 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| subjects | Binomial distribution Cell division correlation analysis Data analysis Developmental stages Females Flowers Genes Genetic aspects Genomes Identification and classification MicroRNA MicroRNAs miRNA Physiological aspects pistil Pistils Plant growth Pomegranate Proteins Regulation RNA polymerase Software |
| title | Exploring MicroRNAs Associated with Pomegranate Pistil Development: An Identification and Analysis Study |
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