Rumi and Pasteurized Kareish Cheeses Are a Source of β-Lactam-Resistant Salmonella in the Nile Delta Region of Egypt: Insights into Their Incidence, AMR Pattern, Genotypic Determinants of Virulence and β-Lactam Resistance

The spread of superbugs in dairy products can jeopardize global public health. To date, information on the incidence rates of virulent and β-lactams-resistant (BLR) in cheeses from rural areas of Egypt has been lacking. Biochemical, serological, antibiotic susceptibility, and multiplex PCR (M-PCR) t...

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Bibliographic Details
Published in:Antibiotics (Basel) 2024-05, Vol.13 (5), p.454
Main Authors: Elzhraa, Fatma, Al-Ashmawy, Maha, El-Sherbini, Mohammed, El-Sebaey, Ahmed M, Mohácsi-Farkas, Csilla, Kiskó, Gabriella, Belák, Ágnes
Format: Article
Language:English
Subjects:
Amides
Antibiotics
Antimicrobial agents
antimicrobial resistance
Ceftazidime
Cephalothin
Cheese
Clindamycin
Colistin
Dairy products
Drug resistance
Enzymes
Erythromycin
Food contamination & poisoning
Food safety
Genes
Meropenem
Multidrug resistance
Nile Delta
Pasteurization
pasteurized Kareish cheese
Pathogens
Public health
Rumi cheese
Rural areas
Salmonella
Sanitation
Serotypes
Virulence
virulence genes
Water pollution
β-Lactam antibiotics
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Summary:The spread of superbugs in dairy products can jeopardize global public health. To date, information on the incidence rates of virulent and β-lactams-resistant (BLR) in cheeses from rural areas of Egypt has been lacking. Biochemical, serological, antibiotic susceptibility, and multiplex PCR (M-PCR) tests were performed to identify and characterize isolates. In this study, 44 (15.71%) isolates of eight different serotypes were recovered from 280 samples of Rumi and pasteurized Kariesh cheeses across the Nile Delta region of Egypt. The most predominant serotypes were . Typhimurium, . Enteritidis, and . Infantis. The virulence genes ( , , and ) were identified in all isolates. However, was only detected in . Typhimurium. The highest resistance was developed against Erythromycin and Clindamycin (90.91%), followed by Ceftazidime and Cephalothin (84.09%). Meropenem and colistin were the most effective antibiotics. A high proportion (79.55%) of multi-drug resistance (MDR) isolates carried narrow spectrum (NS), extended-spectrum (ES), and AmpC-BLR genes. The , , , , and BLR genes were positive in 37.04%, 29.63%, 25.93%, 14.81%, 37.04%, and 3.70% of isolates, respectively. In conclusion, a high prevalence of virulence and BLR genes harboring strains in Egyptian cheeses is considered a great threat to public health.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics13050454