Obesity, but not high-fat diet, is associated with bone loss that is reversed via CD4+CD25+Foxp3+ Tregs-mediated gut microbiome of non-obese mice

• Published in: NPJ science of food 2023-04, Vol.7 (1), p.14-14, Article 14
• Main Authors: Song, Wei, Sheng, Qinglin, Bai, Yuying, Li, Li, Ning, Xin, Liu, Yangeng, Song, Chen, Wang, Tianyi, Dong, Xiaohua, Luo, Yane, Hu, Jinhong, Zhu, Lina, Cui, Xiaole, Chen, Bing, Li, Lingling, Cai, Congli, Cui, Haobo, Yue, Tianli
• Format: Article
• Language: English
• Subjects: 631/250/249; 631/326/2565/2134; 692/4020/2741/2135; 692/700/2817; Animal models; Body weight; Bone density; Bone loss; Bone mass; Bone strength; Cancellous bone; CD25 antigen; CD4 antigen; Cell differentiation; Cell proliferation; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Cortical bone; Diet; Fatty acids; Food Microbiology; Food Science; Foxp3 protein; Fragility; High fat diet; Histones; Intestinal microflora; Lymphocytes; Lymphocytes T; Macrophages; Nutrition; Obesity; Osteoclastogenesis; Osteoporosis; Osteoprogenitor cells; Osteoprotegerin; Tibia
• ISSN: 2396-8370; 2396-8370
• DOI: 10.1038/s41538-023-00190-6

Osteoporosis is characterized by decreased bone mass, microarchitectural deterioration, and increased bone fragility. High-fat diet (HFD)-induced obesity also results in bone loss, which is associated with an imbalanced gut microbiome. However, whether HFD-induced obesity or HFD itself promotes osteoclastogenesis and consequent bone loss remains unclear. In this study, we developed HFD-induced obesity (HIO) and non-obesity (NO) mouse models to evaluate the effect of HFD on bone loss. NO mice were defined as body weight within 5% of higher or lower than that of chow diet fed mice after 10 weeks HFD feeding. NO was protected from HIO-induced bone loss by the RANKL /OPG system, with associated increases in the tibia tenacity, cortical bone mean density, bone volume of cancellous bone, and trabecular number. This led to increased bone strength and improved bone microstructure via the microbiome-short-chain fatty acids (SCFAs) regulation. Additionally, endogenous gut-SCFAs produced by the NO mice activated free fatty acid receptor 2 and inhibited histone deacetylases, resulting in the promotion of Treg cell proliferation in the HFD-fed NO mice; thereby, inhibiting osteoclastogenesis, which can be transplanted by fecal microbiome. Furthermore, T cells from NO mice retain differentiation of osteoclast precursors of RAW 264.7 macrophages ex vivo. Our data reveal that HFD is not a deleterious diet; however, the induction of obesity serves as a key trigger of bone loss that can be blocked by a NO mouse-specific gut microbiome.