Metabolism of linoleic acid in porcine epidermis

The time course of linoleic acid metabolism in porcine epidermis has been studied in order to better evaluate the lipid transformations that accompany differentiation in this tissue. One day after intradermal injection of [U-14C]linoleic acid, most of the radioactivity in the epidermis was associate...

Full description

Saved in:
Bibliographic Details
Published in:Journal of lipid research 1990-10, Vol.31 (10), p.1839-1844
Main Authors: Wertz, P W, Downing, D T
Format: Article
Language:English
Subjects:
Animals
Chromatography, Thin Layer
Epidermis - metabolism
Injections, Intradermal
Kinetics
Linoleic Acids - administration & dosage
Linoleic Acids - metabolism
Lipid Metabolism
Lipids - analysis
Swine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The time course of linoleic acid metabolism in porcine epidermis has been studied in order to better evaluate the lipid transformations that accompany differentiation in this tissue. One day after intradermal injection of [U-14C]linoleic acid, most of the radioactivity in the epidermis was associated with phospholipids, triglycerides, and free fatty acids. Within 3-7 days, a portion of the radiolabel was selectively transferred to acylglucosylceramide and acylceramide fractions, after which the specific activities of all lipids fractions decreased. The results suggest that neither the lipoxygenation of linoleate nor the hydrolysis of acylceramide to form acylacid and free sphingosine are major routes of linoleate metabolism in normal epidermis. The principal pathway of linoleate metabolism in normal epidermis appears to be transfer from phospholipids to acylglucosylceramide to acylceramide. The triglyceride fraction, although minor in amount, attains an initially high specific activity, and may serve as an intermediate in linoleate transfer.
ISSN:0022-2275
DOI:10.1016/S0022-2275(20)42327-2