Determination of azathioprine in bulk and pharmaceutical dosage form by HPTLC

An HPTLC method for analysis of Azathioprine in bulk and pharmaceutical formulation has been established and validated. The analyte was separated on aluminium plates precoated with silica gel 60 F254. The mobile phase was Ethyl acetate: Methanol: Triethylamine (4:1:0.5v/v). Quantification was done b...

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Bibliographic Details
Published in:Journal of pharmacy & bioallied science 2012-10, Vol.4 (4), p.318-321
Main Authors: Jain, Pritam S, Thakre, Pradip, Chaudhari, Amar J, Chavhan, Manish L, Surana, Sanjay J
Format: Article
Language:English
Subjects:
Analytical chemistry
Azathioprine
Calibration
Chromatography
Drug dosages
Environmental conditions
HPTLC
Identification and classification
Measurement
Methods
Original
Pharmaceutical chemistry
Pharmaceuticals
Regression analysis
Standard deviation
validation
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Summary:An HPTLC method for analysis of Azathioprine in bulk and pharmaceutical formulation has been established and validated. The analyte was separated on aluminium plates precoated with silica gel 60 F254. The mobile phase was Ethyl acetate: Methanol: Triethylamine (4:1:0.5v/v). Quantification was done by densitometric scanning at 300nm. Response was a linear function of Azathioprine concentration in the range of 200-1200 ng/band. The limit of detection and quantification for Azathioprine were 18.58 and 59.14 ng/band, respectively. Average recovery was 100.1% which shows that the method was free from interference from excipients present in the formulation. The established method enabled accurate, precise, and rapid analysis of Azathioprine in bulk as well as pharmaceutical formulation.
ISSN:0976-4879
0975-7406
0975-7406
0976-4879
DOI:10.4103/0975-7406.103263