Plasmacytoid dendritic cells appear inactive during sub-microscopic Plasmodium falciparum blood-stage infection, yet retain their ability to respond to TLR stimulation

Plasmacytoid dendritic cells (pDC) are activators of innate and adaptive immune responses that express HLA-DR, toll-like receptor (TLR) 7, TLR9 and produce type I interferons. The role of human pDC in malaria remains poorly characterised. pDC activation and cytokine production were assessed in 59 ma...

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Bibliographic Details
Published in:Scientific reports 2017-06, Vol.7 (1), p.2596-11, Article 2596
Main Authors: Loughland, Jessica R., Minigo, Gabriela, Sarovich, Derek S., Field, Matt, Tipping, Peta E., Montes de Oca, Marcela, Piera, Kim A., Amante, Fiona H., Barber, Bridget E., Grigg, Matthew J., William, Timothy, Good, Michael F., Doolan, Denise L., Engwerda, Christian R., Anstey, Nicholas M., McCarthy, James S., Woodberry, Tonia
Format: Article
Language:English
Subjects:
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13/2
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631/250/2504/133/1412
631/250/255/1629
Adaptive immunity
Adolescent
Adult
Apoptosis
Blood - parasitology
Caspase
Caspase-3
CD123 antigen
CD40 antigen
CD80 antigen
CD86 antigen
Cell Differentiation
Cells, Cultured
Convalescence
Dendritic cells
Dendritic Cells - immunology
Dendritic Cells - parasitology
Erythrocytes
Experimental infection
Female
Gene expression
Histocompatibility antigen HLA
Humanities and Social Sciences
Humans
Immune response
Infections
Interferon
Life Cycle Stages
Malaria
Malaria, Falciparum - immunology
Male
Middle Aged
multidisciplinary
PdC gene
Plasmodium falciparum
Plasmodium falciparum - immunology
Ribonucleic acid
RNA
Science
Science (multidisciplinary)
Sequence Analysis, RNA
T cell receptors
TLR7 protein
TLR9 protein
Toll-Like Receptor 7 - immunology
Toll-Like Receptor 9 - immunology
Toll-like receptors
Transcription
Transcriptome
Vector-borne diseases
Young Adult
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Summary:Plasmacytoid dendritic cells (pDC) are activators of innate and adaptive immune responses that express HLA-DR, toll-like receptor (TLR) 7, TLR9 and produce type I interferons. The role of human pDC in malaria remains poorly characterised. pDC activation and cytokine production were assessed in 59 malaria-naive volunteers during experimental infection with 150 or 1,800 P. falciparum- parasitized red blood cells. Using RNA sequencing, longitudinal changes in pDC gene expression were examined in five adults before and at peak-infection. pDC responsiveness to TLR7 and TLR9 stimulation was assessed in-vitro . Circulating pDC remained transcriptionally stable with gene expression altered for 8 genes (FDR 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-02096-2