UBE2E3 regulates cellular senescence and osteogenic differentiation of BMSCs during aging

Osteoporosis has gradually become a public health problem in the world. However, the exact molecular mechanism of osteoporosis still remains unclear. Senescence and osteogenic differentiation inhibition of bone marrow mesenchymal stem cells (BMSCs ) are supposed to play an important part in osteopor...

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Published in:PeerJ (San Francisco, CA) CA), 2021-11, Vol.9, p.e12253-e12253, Article e12253
Main Authors: Liu, Yalin, Cai, Guangping, Chen, Peng, Jiang, Tiejian, Xia, Zhuying
Format: Article
Language:English
Subjects:
Aging
Bioinformatics
Biotechnology
Bone marrow
Cell differentiation
Data Mining and Machine Learning
Enzymes
Fractures
Gene expression
Genes
Genetic aspects
Genomes
Geriatrics
Laboratory animals
Membranes
Mesenchyme
Metabolic Sciences
Nrf2
Older people
Orthopedics
Osteogenesis
Osteogenic differentiation
Osteoporosis
Proteins
Public health
Senescence
Stem cells
UBE2E3
Ubiquitin
Ubiquitin-conjugating enzyme
Ubiquitin-protein ligase
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Summary:Osteoporosis has gradually become a public health problem in the world. However, the exact molecular mechanism of osteoporosis still remains unclear. Senescence and osteogenic differentiation inhibition of bone marrow mesenchymal stem cells (BMSCs ) are supposed to play an important part in osteoporosis. We used two gene expression profiles (GSE35956 and GSE35958) associated with osteoporosis and selected the promising gene Ubiquitin-conjugating enzyme E2 E3 (UBE2E3). We then verified its function and mechanism by experiments. UBE2E3 was highly expressed in the bone marrow and positively associated with osteogenesis related genes. Besides, UBE2E3 expression reduced in old BMSCs compared with that in young BMSCs. In experiments, knockdown of UBE2E3 accelerated cellular senescence and inhibited osteogenic differentiation of young BMSCs. On the other hand, overexpression of UBE2E3 attenuated cellular senescence as well as enhanced osteogenic differentiation of old BMSCs. Mechanistically, UBE2E3 might regulate the nuclear factor erythroid 2-related factor (Nrf2) and control its function, thus affecting the senescence and osteogenic differentiation of BMSCs. UBE2E3 may be potentially involved in the pathogenesis of osteoporosis by regulating cellular senescence and osteogenic differentiation of BMSCs.
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.12253