Retroperitoneal white adipose tissue lipoprotein lipase activity is rapidly down-regulated in response to acute stress

Tissue-specific regulation of LPL has been widely studied in rats. Previous studies reported that in vivo administration of adrenaline and acute stress cause an increase in plasma LPL activity coinciding with a decrease in white adipose tissue (WAT) LPL activity. We studied the speed of LPL activity...

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Bibliographic Details
Published in:Journal of lipid research 2007-04, Vol.48 (4), p.863-868
Main Authors: Casanovas, Albert, Parramon, Núria, de la Cruz, Ferran, Andrés, Olga, Terencio, José, López-Tejero, M. Dolores, Llobera, Miquel
Format: Article
Language:English
Subjects:
Adipose Tissue, White - enzymology
Animals
Biological Transport
blood pressure
Down-Regulation
fight-or-flight
heart rate
Immobilization
Intra-Abdominal Fat - enzymology
Kinetics
Lipoprotein Lipase - blood
Lipoprotein Lipase - metabolism
plasma
rat
Rats
Stress, Physiological - metabolism
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Summary:Tissue-specific regulation of LPL has been widely studied in rats. Previous studies reported that in vivo administration of adrenaline and acute stress cause an increase in plasma LPL activity coinciding with a decrease in white adipose tissue (WAT) LPL activity. We studied the speed of LPL activity changes during 30 min of stress by immobilization (IMMO) in rats. A first experimental approach in permanently cannulated rats permitted sequential blood sampling in the same animal during IMMO and the obtaining of hemodynamic parameters. In a second experimental approach, animals were euthanized at different times after the start of IMMO to determine LPL activity in tissues. Stress was characterized by rises in blood pressure, heart rate, plasma corticosterone, and available circulating energy substrates. Five min after the start of IMMO, LPL activity fell in retroperitoneal WAT and increased in plasma. These data show the quickest LPL activity change ever described in response to a physiological situation. The speed and simultaneity of these changes suggest that the release from endothelium to the bloodstream may constitute a fast nonexplored mechanism of tissue LPL activity regulation, involved in the lipid energy-substrate redistribution between tissues needed to prepare the “fight-or-flight” response.
ISSN:0022-2275
1539-7262
DOI:10.1194/jlr.M600487-JLR200