Induction of Monoterpenoid Oxindole Alkaloids Production and Related Biosynthetic Gene Expression in Response to Signaling Molecules in Hamelia patens Plant Cultures

(Rubiaceae), known as firebush, is a source of bioactive monoterpenoid oxindole alkaloids (MOAs) derived from monoterpenoid indole alkaloids (MIAs). With the aim of understanding the regulation of the biosynthesis of these specialized metabolites, micropropagated plants were elicited with jasmonic a...

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Published in:Plants (Basel) 2024-03, Vol.13 (7), p.966
Main Authors: López-Vázquez, Ana Luisa, Sepúlveda-García, Edgar Baldemar, Rubio-Rodríguez, Elizabeth, Ponce-Noyola, Teresa, Trejo-Tapia, Gabriela, Barrera-Cortés, Josefina, Cerda-García-Rojas, Carlos M, Ramos-Valdivia, Ana C
Format: Article
Language:English
Subjects:
Alkaloids
Biosynthesis
Cloning
Enzymes
Gene expression
Genes
Hamelia
Hamelia patens
indoles
Jasmonic acid
Metabolites
micropropagation
monoterpene indole alkaloids
monoterpenoids
Oxindole alkaloids
Salicylic acid
tetrahydroalstonine synthase
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Summary:(Rubiaceae), known as firebush, is a source of bioactive monoterpenoid oxindole alkaloids (MOAs) derived from monoterpenoid indole alkaloids (MIAs). With the aim of understanding the regulation of the biosynthesis of these specialized metabolites, micropropagated plants were elicited with jasmonic acid (JA) and salicylic acid (SA). The MOA production and MIA biosynthetic-related gene expression were evaluated over time. The production of MOAs was increased compared to the control up to 2-fold (41.3 mg g DW ) at 72 h in JA-elicited plants and 2.5-fold (42.4 mg g DW ) at 120 h in plants elicited with SA. The increment concurs with the increase in the expression levels of the genes , , , , , and Interestingly, it was found that was downregulated in both treatments after 24 h but in the SA treatment at 120 h only was upregulated to 8-fold compared to the control. In this work, we present the results of MOA production in and discuss how JA and SA might be regulating the central biosynthetic steps that involve and genes.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants13070966