Inhibitor of DNA binding 2 accelerates nerve regeneration after sciatic nerve injury in mice

Inhibitor of DNA binding 2 (Id2) can promote axonal regeneration after injury of the central nervous system. However, whether Id2 can promote axonal regeneration and functional recovery after peripheral nerve injury is currently unknown. In this study, we established a mouse model of bilateral sciat...

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Bibliographic Details
Published in:Neural regeneration research 2021-12, Vol.16 (12), p.2542-2548
Main Authors: Huang, Zhong-Hai, Feng, Ai-Ying, Liu, Jing, Zhou, Libing, Zhou, Bing, Yu, Panpan
Format: Article
Language:English
Subjects:
Analysis
axonal regeneration
functional recovery
inhibitor of dna binding 2
motor neuron
neuromuscular junctions
peripheral nerve
reinnervation
sciatic nerve injury
Diseases
DNA-ligand interactions
Rodents
Sciatic nerve
Spinal cord
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Summary:Inhibitor of DNA binding 2 (Id2) can promote axonal regeneration after injury of the central nervous system. However, whether Id2 can promote axonal regeneration and functional recovery after peripheral nerve injury is currently unknown. In this study, we established a mouse model of bilateral sciatic nerve crush injury. Two weeks before injury, AAV9-Id2-3×Flag-GFP was injected stereotaxically into the bilateral ventral horn of lumbar spinal cord. Our results showed that Id2 was successfully delivered into spinal cord motor neurons projecting to the sciatic nerve, and the number of regenerated motor axons in the sciatic nerve distal to the crush site was increased at 2 weeks after injury, arriving at the tibial nerve and reinnervating a few endplates in the gastrocnemius muscle. By 1 month after injury, extensive neuromuscular reinnervation occurred. In addition, the amplitude of compound muscle action potentials of the gastrocnemius muscle was markedly recovered, and their latency was shortened. These findings suggest that Id2 can accelerate axonal regeneration, promote neuromuscular reinnervation, and enhance functional improvement following sciatic nerve injury. Therefore, elevating the level of Id2 in adult neurons may present a promising strategy for peripheral nerve repair following injury. The study was approved by the Experimental Animal Ethics Committee of Jinan University (approval No. 20160302003) on March 2, 2016.
ISSN:1673-5374
1876-7958
DOI:10.4103/1673-5374.313054