Modular barcode beads for microfluidic single cell genomics

Barcode beads allow efficient nucleic acid tagging in single cell genomics. Current barcode designs, however, are fabricated with a particular application in mind. Repurposing to novel targets, or altering to add additional targets as information is obtained is possible but the result is suboptimal....

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Bibliographic Details
Published in:Scientific reports 2021-05, Vol.11 (1), p.10857-10857, Article 10857
Main Authors: Delley, Cyrille L., Abate, Adam R.
Format: Article
Language:English
Subjects:
631/1647/2163
631/1647/2217
631/1647/2217/2018
631/208/69
631/337/2019
Biomarkers, Tumor
DNA Barcoding, Taxonomic
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Genomics
Genomics - methods
Humanities and Social Sciences
Humans
Microfluidics
Microfluidics - methods
multidisciplinary
Neoplasms - etiology
Nucleic acids
Science
Science (multidisciplinary)
Single-Cell Analysis - methods
Transcriptome
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Description
Summary:Barcode beads allow efficient nucleic acid tagging in single cell genomics. Current barcode designs, however, are fabricated with a particular application in mind. Repurposing to novel targets, or altering to add additional targets as information is obtained is possible but the result is suboptimal. Here, we describe a modular framework that simplifies generation of multifunctional beads and allows their easy extension to new targets.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-90255-x