Lipopolysaccharide stimulation test on cultured PBMCs assists the discrimination of cryopyrin-associated periodic syndrome from systemic juvenile idiopathic arthritis

Systemic juvenile idiopathic arthritis (sJIA) and cryopyrin-associated periodic syndrome (CAPS) share many common manifestations. We aim to identify an applicable method to assist disease discrimination. Inflammatory cytokines were measured in the plasma of patients with CAPS, sJIA with persistent d...

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Published in:Scientific reports 2021-06, Vol.11 (1), p.11903-11903, Article 11903
Main Authors: Wu, Chao-Yi, Fan, Wen-Lang, Chiu, Ying-Ming, Yang, Huang-Yu, Lee, Wen-I., Huang, Jing-Long
Format: Article
Language:English
Subjects:
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Arthritis
ATP
Caspase-1
Cell activation
Cryopyrin
Cytokines
Humanities and Social Sciences
IL-1β
Inflammasomes
Interleukin 18
Leukocytes (mononuclear)
Lipopolysaccharides
Macrophages
multidisciplinary
Peripheral blood mononuclear cells
Science
Science (multidisciplinary)
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Summary:Systemic juvenile idiopathic arthritis (sJIA) and cryopyrin-associated periodic syndrome (CAPS) share many common manifestations. We aim to identify an applicable method to assist disease discrimination. Inflammatory cytokines were measured in the plasma of patients with CAPS, sJIA with persistent disease course and healthy controls. Supernatants collected from non-stimulated peripheral blood mononuclear cells (PBMCs) and those undergone inflammasome stimulation tests utilizing lipopolysaccharide (LPS) with and without adenosine triphosphate (ATP) were investigated. Inflammatory cytokines in patient plasma fail to differentiate sJIA from CAPS. PBMCs from sJIA secrets higher amount of IL-1β and IL-18 while CAPS PBMCs produces more caspase-1 without stimulation. IL-1β, IL-18, and caspase-1 were significantly elevated among CAPS PBMCs (all p  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-91354-5