Investigating the role of G-quadruplexes at Saccharomyces cerevisiae telomeres

The G-quadruplex consensus motif G≥3NxG≥3NxG≥3NxG≥3 is found at telomeres of many species, ranging from yeast to plants to humans, but the biological significance of this fact remains largely unknown. In this study, we examine the in vivo relevance of telomeric G-quadruplexes in the budding yeast Sa...

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Bibliographic Details
Published in:Microbial cell 2022-06, Vol.9 (6), p.126-132
Main Authors: Stinus, Sonia, Rosas Bringas, Fernando R., Wanders, Lisa, Chang, Michael
Format: Article
Language:English
Subjects:
Budding Yeast
cdc13
G-Quadruplex
pif1
Research Report
Saccharomycotina
Telomere
tlc1-tm
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Summary:The G-quadruplex consensus motif G≥3NxG≥3NxG≥3NxG≥3 is found at telomeres of many species, ranging from yeast to plants to humans, but the biological significance of this fact remains largely unknown. In this study, we examine the in vivo relevance of telomeric G-quadruplexes in the budding yeast Saccharomyces cerevisiae by expressing a mutant telomerase RNA sub- unit (tlc1-tm) that introduces mutant [(TG)0–4TGG]xATTTGG telomeric repeats instead of wild-type (TG)0-6TGGGTGTG(G)0-1 repeats to the distal ends of telo- meres. The tlc1-tm telomere sequences lack the GGG motif present in every wild-type repeat and, therefore, are expected to be impaired in the formation of G-quadruplexes. Circular dichroism analysis of oligonucleotides consisting of tlc1-tm telomeric sequence is consistent with this hypothesis. We have previously shown that tlc1-tm cells grow similarly to wild-type cells, suggest- ing that the ability to form telomeric G-quadruplexes is not essential for te- lomere capping in S. cerevisiae cells.
ISSN:2311-2638
2311-2638
DOI:10.15698/mic2022.06.778