Development of Microsatellite Loci for Cyclocarya paliurus (Juglandaceae), A Monotypic Species in Subtropical China

Premise of the study: Microsatellite loci were developed for a monotypic species endemic to subtropical China, Cyclocarya paliurus, to help infer the evolutionary histories of ancient monotypic genera in subtropical China. Methods and Results: Using the Fast Isolation by AFLP of Sequences Containing...

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Bibliographic Details
Published in:Applications in plant sciences 2013-06, Vol.1 (6), p.1200524-n/a
Main Authors: Fan, Deng-Mei, Ye, Lin-Jiang, Luo, Yi, Hu, Wan, Tian, Shuang, Zhang, Zhi-Yong
Format: Article
Language:English
Subjects:
alleles
Amplified fragment length polymorphism
China
Cloning
Cyclocarya paliurus
Deoxyribonucleic acid
DNA
DNA primers
Endemic species
Evolution
Genetic analysis
Genetic diversity
genetic variation
indigenous species
Juglandaceae
Juglans regia
loci
microsatellite marker
microsatellite repeats
phylogeography
Plant sciences
Platycarya
Polymorphism
Population
Population genetics
PRIMER NOTE
Pterocarya
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Summary:Premise of the study: Microsatellite loci were developed for a monotypic species endemic to subtropical China, Cyclocarya paliurus, to help infer the evolutionary histories of ancient monotypic genera in subtropical China. Methods and Results: Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, 28 primer sets were identified in two wild populations. All loci were polymorphic, with the number of alleles per locus ranging from two to eight. The expected and observed heterozygosities ranged from 0.153 to 0.802 and from 0 to 0.750, respectively. The transferability of the 28 primer pairs was tested on Juglans regia, Pterocarya stenoptera, and Platycarya strobilacea. Twenty-one (75.0%), 22 (78.6%), and 15 (53.6%) markers were successfully amplified in J. regia, P. stenoptera, and P. strobilacea, respectively. Conclusions: These loci will be useful for in-depth analysis of genetic diversity and phylogeographical variation throughout the distribution range of C. paliurus.
ISSN:2168-0450
2168-0450
DOI:10.3732/apps.1200524