Fecal Proteomic Analysis in Healthy Dogs and in Dogs Suffering from Food Responsive Diarrhea

Different laboratory markers are routinely used in the diagnosis and management of gastrointestinal (GI) disease in dogs. In the present study, starting from feces from both healthy dogs and dogs suffering from food responsive diarrhea (FRD), we tried to find proteins differently expressed in the tw...

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Bibliographic Details
Published in:TheScientificWorld 2019, Vol.2019 (2019), p.1-7
Main Authors: Ricciutelli, Massimo, Marchegiani, Andrea, Vincenzetti, Silvia, Scortichini, Luca, Pucciarelli, Stefania, Pengo, Graziano, Gavazza, Alessandra, Tesei, Beniamino, Spaterna, A., Rossi, Giacomo, Cerquetella, Matteo, Sagratini, Gianni
Format: Article
Language:English
Subjects:
Animal Feed - microbiology
Animals
Chains
Chromatography, Liquid
Diagnostic systems
Diarrhea
Diarrhea - veterinary
Dimers
Disease control
Dog Diseases - epidemiology
Dog Diseases - metabolism
Dog Diseases - microbiology
Dogs
Feces
Feces - chemistry
Fluids
Food
Foodborne Diseases - veterinary
Immune system
Immunoglobulin A
Immunology
Inflammatory bowel disease
Laboratories
Monomers
Mucosa
Mucosal immunity
Proteins
Proteome
Proteomics
Proteomics - methods
Secretions
Tandem Mass Spectrometry
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Summary:Different laboratory markers are routinely used in the diagnosis and management of gastrointestinal (GI) disease in dogs. In the present study, starting from feces from both healthy dogs and dogs suffering from food responsive diarrhea (FRD), we tried to find proteins differently expressed in the two groups of dogs, by using a proteomic approach. Interestingly, we found that the immunoglobulin J-chain isoform 1 (species: Canis lupus familiaris) was identified only in diseased dogs (not in healthy). J-chain combines especially IgA monomers to IgA dimers and plays a crucial role for their secretions into mucosal interface. Being the first study of that kind in the dog, it is only possible to hypothesize that their presence could be likely due to an increased activation of the immune system or to a mucosal damage or both in FRD patients. Similarly, it is still impossible to assess whether this protein could be used as diagnostic/prognostic marker of GI disease; however, this study represents a promising first step toward fecal proteomics in canine GI disorders.
ISSN:2356-6140
1537-744X
DOI:10.1155/2019/2742401