A double-blind intervention trial in healthy women demonstrates the beneficial impact on Bifidobacterium with low dosages of prebiotic galacto-oligosaccharides

• Published in: Frontiers in nutrition (Lausanne) 2024-08, Vol.11, p.1440319
• Main Authors: Looijesteijn, Ellen, Schoemaker, Marieke H, van den Belt, Maartje, Hester, Eric R, Kortman, Guus A M, Viskaal-van Dongen, Mirre, Nauta, Arjen
• Format: Article
• Language: English
• Subjects: Bifidobacterium; galacto-oligosaccharides; GOS; gut microbiota; Nutrition; prebiotic; supplement
• ISSN: 2296-861X; 2296-861X
• DOI: 10.3389/fnut.2024.1440319

Galacto-oligosaccharides (GOS) are well-substantiated prebiotic substrates. Multiple studies have demonstrated a positive impact of GOS on gut microbiota composition and activity, so-far mainly related to . However, data on the beneficial impact at lower dosages in a healthy female population are limited. The primary aim of the current study was to reveal the effect of low dosages (1.3 and 2.0 g) of GOS on fecal abundance in healthy women. Other outcomes included the effect of low dosage of GOS on overall fecal microbiota composition and on self-perceived GI comfort, sleep quality and mental wellbeing. Eighty-eight healthy women (42-70 years, BMI 18.7-30 kg/m ) were included in this randomized, parallel, double-blind study of 6 weeks. The participants were stratified for fiber intake, BMI and age and randomized to consume either 1.3 or 2.0 g of GOS per day for 3 weeks after a control period of 3 weeks without any intervention. Fecal samples were collected for shotgun metagenomics sequencing at the start (t = -3) and end (t = 0) of the control period and at the end of the intervention period (t = 3). Self-perceived gut comfort, sleep quality, and mental wellbeing were assessed weekly. Hierarchical clustering of principal components was applied to data collected from study participants. The relative abundance of in feces increased significantly after 3 weeks of daily consumption of both 1.3 g (