Commercial microbiota test revealed differences in the composition of intestinal microorganisms between children with autism spectrum disorders and neurotypical peers

• Published in: Scientific reports 2021-12, Vol.11 (1), p.24274-11, Article 24274
• Main Authors: Jendraszak, Magdalena, Gałęcka, Mirosława, Kotwicka, Małgorzata, Regdos, Aleksandra, Pazgrat-Patan, Michalina, Andrusiewicz, Mirosław
• Format: Article
• Language: English
• Subjects: 631/1647/2234; 631/326/2522; 692/4020; 692/617/375/366/1373; Akkermansia; Akkermansia muciniphila; Allergies; Autism; Autism Spectrum Disorder - microbiology; Autistic children; Bacteroides; Bifidobacterium; Body Mass Index; Child; Child, Preschool; Children; Cognitive ability; E coli; Enterococcus; Escherichia coli; Faecalibacterium; Feces; Female; Gastrointestinal Microbiome - genetics; Humanities and Social Sciences; Humans; Hypersensitivity; Inflammation; Intestinal microflora; Intestine; Intestines - microbiology; Klebsiella; Lactobacillus; Male; Microbiology; Microbiota; Microorganisms; multidisciplinary; Principal Component Analysis; Probiotics; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-03794-8

The early-life modifications of intestinal microbiota may impact children's subsequent emotional and cognitive development. Studies show that some bacteria species in gut microbiota, and the lack of others, may play a key role in autism spectrum disorders (ASD) development. Fecal samples were obtained from three groups of children: 16 healthy, 24 with allergies (ALG), and 33 with ASD (probiotics and non-probiotics users). The analysis was carried out according to the KyberKompakt Pro protocol. We observed a significantly higher level of Klebsiella spp. in the healthy children from the non-probiotics group, considering three groups. In the same group, Bifidobacterium spp . the level was lower in ASD compared to neurotypical individuals. In healthy children who did not use probiotics, strong positive correlations were observed in E. coli and Enterococcus spp . and Bacteroides and Klebsiella spp., and a negative correlation for Akkermansia muciniphila with both Klebsiella spp . and Bacteroides spp . In the ASD group who take probiotics, a strongly negative correlation was observed in Lactobacillus spp., and both Faecalibacterium prausnitzii and Akkermansia muciniphila levels . In the ALG group, the strongest, negative correlation was found between Enterococcus spp . and Lactobacillus spp . as in Akkermansia muciniphila and Bifidobacterium spp. The simple commercial test revealed minor differences in the composition of intestinal microorganisms between children with autism spectrum disorders and neurotypical peers.