Polyamidoamide Dendrimers and Cross-Linking Agents for Stabilized Bioenzymatic Resistant Metal-Free Bovine Collagen

The work reports the use of polyamidoamine dendrimers (PAMAM) and a cross-linking agent, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/ -hydroxysuccinimide (EDC/NHS) or 4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM), for the thermal stabilization of dermal bovine collag...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2019-10, Vol.24 (19), p.3611
Main Authors: Beghetto, Valentina, Gatto, Vanessa, Conca, Silvia, Bardella, Noemi, Scrivanti, Alberto
Format: Article
Language:English
Subjects:
Animals
Calorimetry
Calorimetry, Differential Scanning
Carbodiimide
Cattle
Collagen
Collagen - chemistry
Collagenases - metabolism
cross-linking
Cross-Linking Reagents - chemistry
Crosslinking
Degradability
dendrimer
Dendrimers
Dendrimers - chemistry
Differential scanning calorimetry
dmtmm
edc
enzymatic degradation
Enzyme Assays
Experiments
Hides
Laboratories
Metals
Molecular weight
pamam
Phenols
Proteins
Succinimides - chemistry
Tanning
Temperature
Thermal stability
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Summary:The work reports the use of polyamidoamine dendrimers (PAMAM) and a cross-linking agent, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/ -hydroxysuccinimide (EDC/NHS) or 4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM), for the thermal stabilization of dermal bovine collagen. The efficiency of EDC/NHS/PAMAM and DMTMM/PAMAM in the cross-linking of collagen is correlated to the increase of the collagen shrinkage temperature (Ts), measured by differential scanning calorimetry (DSC). An alternative enzymatic protocol was adopted to measure the degradability of EDC/NHS/PAMAM tanned hides; these data are correlated to the thermal stability values measured by DSC. In the presence of PAMAMs, EDC/NHS provides very high stabilization of bovine dermal collagen, giving Ts of up to 95 °C, while DMTMM achieves lower stabilization. Preliminary tanning tests carried out in best reaction conditions show that EDC/NHS/PAMAM could be an interesting, environmentally-sustainable tanning system which is completely free of metals, formaldehyde, and phenols. Two new unreported dendrimeric species were synthesized and employed.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24193611