MicroRNA profiling of different exercise interventions for alleviating skeletal muscle atrophy in naturally aging rats

Background Exercise is an affordable and practical strategy to alleviate several detrimental outcomes from the aging process, including sarcopenia. The elucidation of molecular mechanisms to alleviate sarcopenia is one of the most important steps towards understanding human aging. Although microRNAs...

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Bibliographic Details
Published in:Journal of cachexia, sarcopenia and muscle sarcopenia and muscle, 2023-02, Vol.14 (1), p.356-368
Main Authors: Liang, Jiling, Zhang, Hu, Zeng, Zhengzhong, Lv, Jun, Huang, Jielun, Wu, Xiaowen, Wang, Minghui, Xu, Jiahao, Fan, Jingjing, Chen, Ning
Format: Article
Language:English
Subjects:
Aerobics
Aging
Aging - genetics
Aging - metabolism
Animals
Atrophy
Biomarkers - metabolism
Data analysis
Exercise
exercise intervention
Fitness equipment
Fitness training programs
Genes
Male
Metabolism
microRNA
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA sequencing
Muscle, Skeletal - pathology
Muscular Atrophy - genetics
Muscular Atrophy - metabolism
Muscular Atrophy - therapy
Musculoskeletal system
NF-kappa B - metabolism
Older people
Original
Oxidative stress
Pathogenesis
Physical Conditioning, Animal
Physical fitness
Proteins
Rats
Running
Sarcopenia
Sarcopenia - genetics
Sarcopenia - metabolism
Sarcopenia - therapy
skeletal muscle atrophy
Software
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Summary:Background Exercise is an affordable and practical strategy to alleviate several detrimental outcomes from the aging process, including sarcopenia. The elucidation of molecular mechanisms to alleviate sarcopenia is one of the most important steps towards understanding human aging. Although microRNAs (miRNAs) regulate muscle growth, regeneration and aging, the potential role of exercise‐mediated miRNAs during the prevention and rehabilitation of skeletal muscle atrophy upon exercise interventions remains unclear. Methods A miRNA profile by miRNA sequencing for gastrocnemius muscle of a 24‐month‐old aged male rat model mimicking the naturally aging process was established through screening the differentially expressed miRNAs (DEMs) for alleviating aging‐induced skeletal muscle atrophy upon optimal exercise intervention. The screened miRNAs and hub genes, as well as biomarkers with the most significantly enriched pathways, were validated by quantitative real‐time polymerase chain reaction and western blotting. Results The sarcopenia index (SI) value and cross‐sectional area (CSA) of rats from the old control (OC) group significantly decreased when compared with the youth control (YC) group (P 
ISSN:2190-5991
2190-6009
DOI:10.1002/jcsm.13137