Loss of Ripk3 attenuated neutrophil accumulation in a lipopolysaccharide-induced zebrafish inflammatory model

Neutrophils are important effector cells during inflammation, which play complex roles. Therefore, investigating the regulation of neutrophil accumulation during inflammation might provide targets for treating related diseases. In the present study, we generated a ripk3 -deficient zebrafish line to...

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Bibliographic Details
Published in:Cell death discovery 2022-02, Vol.8 (1), p.88-88, Article 88
Main Authors: Wen, Wanying, Chen, Jiakui, Zhou, Yuxin, Li, Gaofei, Zhang, Yiyue
Format: Article
Language:English
Subjects:
631/250/2504
631/250/256
Apoptosis
Biochemistry
Biomedical and Life Sciences
Cell Biology
Cell Cycle Analysis
Cytokines
Danio rerio
Effector cells
Infectious diseases
Inflammation
Larvae
Leukocytes (neutrophilic)
Life Sciences
Lipopolysaccharides
Neutrophils
Stem Cells
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Summary:Neutrophils are important effector cells during inflammation, which play complex roles. Therefore, investigating the regulation of neutrophil accumulation during inflammation might provide targets for treating related diseases. In the present study, we generated a ripk3 -deficient zebrafish line to study the roles of Ripk3 in neutrophil-related inflammation. The homeostatic hematopoiesis and cytokine expression of the ripk3 -deficient larvae were unaltered. The ripk3 -deficient larvae with caudal fin fold injury exhibited similar neutrophil enrichment with wild-type larvae, suggesting that Ripk3 is not essential for non-infectious inflammatory responses. When challenged with lipopolysaccharide (LPS), the ripk3 -deficient larvae showed significantly less neutrophil accumulation in the injection site and differential expression of several key cytokines. Ripk3 inhibitors could also attenuate neutrophil accumulation in wild-type larvae, indicating that Ripk3 could serve as a candidate target for inflammation treatment. In summary, our study indicated that Ripk3 has an essential role in LPS-induced inflammatory responses. It was suggested that the ripk3 -deficient zebrafish might be applied in developing infectious disease models, while Ripk3 also has potential as an inflammation-treatment target.
ISSN:2058-7716
2058-7716
DOI:10.1038/s41420-022-00891-z