Rapid Fermentable Substance Modulates Interactions between Ruminal Commensals and Toll-Like Receptors in Promotion of Immune Tolerance of Goat Rumen

Whether dietary non-fiber carbohydrate (NFC), a rapid fermentable substance, affects immune homeostasis of rumen through the modulation of interactions of ruminal microbiota and epithelial toll-like receptors ( ) remains unclear. A combination of 16S rRNA amplicon sequencing and quantitative PCRs wa...

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Bibliographic Details
Published in:Frontiers in microbiology 2016-11, Vol.7, p.1812-1812
Main Authors: Shen, Hong, Lu, Zhongyan, Chen, Zhan, Wu, Yufeng, Shen, Zanming
Format: Article
Language:English
Subjects:
dietary modulation
epithelium transport
Immune Tolerance
microbe-host interactions
Microbiology
rumen microbiota
Toll-Like Receptors
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Summary:Whether dietary non-fiber carbohydrate (NFC), a rapid fermentable substance, affects immune homeostasis of rumen through the modulation of interactions of ruminal microbiota and epithelial toll-like receptors ( ) remains unclear. A combination of 16S rRNA amplicon sequencing and quantitative PCRs was applied to study the synergetic responses of ruminal microbiota and epithelial to the dietary NFC switch from 15 to 31% in the goat model. The results showed that the 31% NFC diet caused the radical increases on the richness and diversity of rumen microbiota. The phylum Verrucomicrobia was most significantly expanded, whereas opportunistic pathogens, namely , , and , were significantly decreased. In rumen epithelium, the significantly increased expressions of , , were associated with the significantly decreased expressions of pro-inflammatory cytokines interleukin-1beta ( ), , and anti-inflammatory cytokine . Constrained correlation analysis indicated that the increased abundance of commensal bacteria in Verrucomicrobia subdivision 5 contributed to the upregulation of expression. Finally, the significantly increased concentrations of rumen short-chain fatty acids (SCFAs), coupled with the significantly upregulated expressions of epithelial genes related to SCFA absorption were observed in goats fed with 31% NFC diet. Thus, the NFC-induced expansion of rumen microbiota promoted epithelium tolerance by enhancement of the intensity of signaling. The newly established equilibrium benefited to the transport of ruminal energy substances into the blood.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2016.01812