Microbial analysis reveals the potential colonization of pathogens in the intestine of crayfish (Procambarus clarkii) in traditional aquaculture environments

• Published in: Ecotoxicology and environmental safety 2021-11, Vol.224, p.112705, Article 112705
• Main Authors: Feng, Yang, Li, Minghao, Duan, Huimin, Li, Liangyu, Ouyang, Ping, Chen, Defang, Geng, Yi, Huang, Xiaoli, Yang, Shiyong, Yin, Lizi, Jiang, Jun, Zhang, Xiaoli
• Format: Article
• Language: English
• Subjects: Environment-host interaction; Microbial community; Microbiota analysis; Potential pathogen colonization; Procambarus clarkii; Traditional cultivation pattern
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2021.112705

The microbiota of the intestine produces a wide array of biologically active molecules and together act as a composite endocrine organ. Due to our limited understanding of bacterial communities in aquaculture ecosystems, it is necessary to evaluate the interactions between environmental and intestinal microbiota and the potential consequences of disease. This study taken the traditional P. clarkii culture in the Sichuan Basin as an example, and analyzed the relationships between the microbiota of the environment and host through microbial analysis and microbiological diagnosis. Our results showed that the bacterial abundance in sediment was greater than in water, followed by the intestine, and some of bacteria from the environment successfully selected to colonize the intestine. The bacterial composition in the intestines of diseased and healthy crayfish was significantly different. The bacteria that colonized and proliferated in the intestine had very low abundances in sediment and water. Two potential pathogens, Aeromonas veronii, and Citrobacter freundii, and two potential probiotics, Lactococcus garvieae and Exiguobacterium undae, were identified. Using multiple, real, and traditional P. clarkii aquaculture sites in the Sichuan Basin, this study revealed that the microbial communities of the environment and animal host did indeed interact. Furthermore, these results indicated that P. clarkii in a healthy status are capable of regulating which bacteria colonize their intestines. •The bacterial abundance in sediment was greater than in water, followed by the intestine.•Two bacteria species, L. garvieae and Exiguobacterium, dominated the healthy intestines.•C. bacilloplasma, A. veronii, Citrobacter, and RSAHF231, were dominant in diseased intestines.•Similar microbial community were found in the Sichuan Basin•A. veronii and C. freundii were isolated from parenteral sites of diseased P. clarkia.