GRK6 regulates the hemostatic response to injury through its rate-limiting effects on GPCR signaling in platelets

• Published in: Blood advances 2020-01, Vol.4 (1), p.76-86
• Main Authors: Chen, Xi, Gupta, Shuchi, Cooper, Matthew, DeHelian, Daniel, Zhao, Xuefei, Naik, Meghna U., Wurtzel, Jeremy G.T., Stalker, Timothy J., Goldfinger, Lawrence E., Benovic, Jeffrey, Brass, Lawrence F., McKenzie, Steven E., Naik, Ulhas P., Ma, Peisong
• Format: Article
• Language: English
• Subjects: Animals; Blood Platelets; Hemostatics; Mice; Platelet Activation; Receptors, Thrombin; Signal Transduction; Thrombosis and Hemostasis
• ISSN: 2473-9529; 2473-9537
• DOI: 10.1182/bloodadvances.2019000467

G protein–coupled receptors (GPCRs) mediate the majority of platelet activation in response to agonists. However, questions remain regarding the mechanisms that provide negative feedback toward activated GPCRs to limit platelet activation and thrombus formation. Here we provide the first evidence that GPCR kinase 6 (GRK6) serves this role in platelets, using GRK6−/− mice generated by CRISPR-Cas9 genome editing to examine the consequences of GRK6 knockout on GPCR-dependent signaling. Hemostatic thrombi formed in GRK6−/− mice are larger than in wild-type (WT) controls during the early stages of thrombus formation, with a rapid increase in platelet accumulation at the site of injury. GRK6−/− platelets have increased platelet activation, but in an agonist-selective manner. Responses to PAR4 agonist or adenosine 5′-diphosphate stimulation in GRK6−/− platelets are increased compared with WT littermates, whereas the response to thromboxane A2 (TxA2) is normal. Underlying these changes in GRK6−/− platelets is an increase in Ca2+ mobilization, Akt activation, and granule secretion. Furthermore, deletion of GRK6 in human MEG-01 cells causes an increase in Ca2+ response and PAR1 surface expression in response to thrombin. Finally, we show that human platelet activation in response to thrombin causes an increase in binding of GRK6 to PAR1, as well as an increase in the phosphorylation of PAR1. Deletion of GRK6 in MEG-01 cells causes a decrease in PAR1 phosphorylation. Taken together, these data show that GRK6 regulates the hemostatic response to injury through PAR- and P2Y12-mediated effects, helping to limit the rate of platelet activation during thrombus growth and prevent inappropriate platelet activation. •GRK6 regulates the hemostatic response by limiting platelet activation via thrombin and adenosine 5′-diphosphate.•GRK6 regulates the hemostatic response by reducing PAR1/4- and P2Y12-dependent signaling. [Display omitted]