Colorimetric Assay for Exon 7 SMN1/SMN2 Single Nucleotide Polymorphism Using Gold Nanoprobes

Among them, gold nanoparticles (Au NPs) have received great attention for potential biological analysis, in recent years, due to the Surface Plasmon Resonance (SPR) phenomena, which is responsible for their unique size and shape optical dependent properties. Because of having high order of magnitude...

Full description

Saved in:
Bibliographic Details
Published in:Bioimpacts 2013-01, Vol.3 (4), p.185-194
Main Authors: Ahmadpour-Yazdi, Hossein, Hormozi-Nezhad, Mohammad Reza, Abadi, Ali Reza, Sanati, Mohammad Hossein, Kazemi, Bahram
Format: Article
Language:English
Subjects:
Colleges & universities
Colorimetric
Deoxyribonucleic acid
DNA
Gold
Gold Nanoprobe
Methods
Nanoparticles
Nucleotide Polymorphism (SNP)
Polymerase chain reaction
Proteins
Spinal Muscular Atrophy (SMA)
Survival of Motor Neuron (SMN)
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Among them, gold nanoparticles (Au NPs) have received great attention for potential biological analysis, in recent years, due to the Surface Plasmon Resonance (SPR) phenomena, which is responsible for their unique size and shape optical dependent properties. Because of having high order of magnitude for extinction coefficients, in addition to the dependence of SPR wavelength and intensity on the dielectric constant of the medium and distance between nanoparticles, Au NPs emerged as a promising tool for analysis of biologically active molecules through colorimetric assay.19-21 In particular, spherical Au NPs due to their stability, shape, highly controllable size, and functionalization have been used for detection of DNA, proteins, small molecules, ions and macromolecules.19 Previously, colorimetric detection of DNA via gold nanoparticles, was introduced by Mirkin's group.22-24 In their proposed system, two oligo- modified gold nanoparticles were used for detection through the formation of a polymeric network between DNA nanoprobes and target DNA cross linkers which caused a distinguishable color change (red-to- pinkish/ purple). On the contrary, as the mismatched target detached from the nanoprobes easily, the electrosteric repulsive force of nanoprobes was attenuated by the screening effect of the salt which consequently changed the color of the reaction from red to blue. [...]as salt-induced aggregation, it exhibited a red shift from 526 to 550-630 nm.
ISSN:2228-5652
2228-5660
DOI:10.5681/bi.2013.037