Calcium-binding properties, stability, and osteogenic ability of phosphorylated soy peptide-calcium chelate

Bioactive peptides based on foodstuffs are of particular interest as carriers for calcium delivery due to their safety and high activity. The phosphorylated peptide has been shown to enhance calcium absorption and bone formation. A novel complex of peptide phosphorylation modification derived from s...

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Published in:Frontiers in nutrition (Lausanne) 2023-04, Vol.10, p.1129548-1129548
Main Authors: Kong, Xiao, Xiao, Ziqun, Chen, Yuhang, Du, MengDi, Zhang, Zihui, Wang, Zhenhua, Xu, Bo, Cheng, Yongqiang, Yu, Tianying, Gan, Jing
Format: Article
Language:English
Subjects:
calcium supplement
characterization
Nutrition
osteogenic differentiation
peptide-calcium chelate
phosphorylation
thermal stability
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Summary:Bioactive peptides based on foodstuffs are of particular interest as carriers for calcium delivery due to their safety and high activity. The phosphorylated peptide has been shown to enhance calcium absorption and bone formation. A novel complex of peptide phosphorylation modification derived from soybean protein was introduced, and the mechanism, stability, and osteogenic differentiation bioactivity of the peptide with or without calcium were studied. The calcium-binding capacity of phosphorylated soy peptide (SPP) reached 50.24 ± 0.20 mg/g. The result of computer stimulation and vibration spectrum showed that SPP could chelate with calcium by the phosphoric acid group, carboxyl oxygen of C-terminal Glu, Asp, and Arg, and phosphoric acid group of Ser on the SPP at a stoichiometric ratio of 1:1, resulting in the formation of the complex of ligand and peptide. Thermal stability showed that chelation enhanced peptide stability compared with SPP alone. Additionally, results showed that SPP-Ca could facilitate osteogenic proliferation and differentiation ability. SPP may function as a promising alternative to current therapeutic agents for bone loss.
ISSN:2296-861X
2296-861X
DOI:10.3389/fnut.2023.1129548