Molecular Characterization of Hepatitis B Virus Infection in a Patient with Cutaneous Lupus Erythematosus

Hepatitis B virus (HBV) infection is a serious global health problem. Patients with autoimmune diseases, such as Lupus Erythematosus, are exposed to a higher risk of acquiring infections. In this study, a molecular characterization, genomic investigation of the Hepatitis B virus, polymerase (P) and...

Full description

Saved in:
Bibliographic Details
Published in:Diagnostics (Basel) 2022-11, Vol.12 (11), p.2866
Main Authors: Villano, Umbertina, Mataj, Elida, Dorrucci, Maria, Farchi, Francesca, Pirone, Carmelo, Valdarchi, Catia, Equestre, Michele, Madonna, Elisabetta, Bruni, Roberto, Pisani, Giulio, Martina, Antonio, Simeoni, Matteo, Iaiani, Giancarlo, Ciccozzi, Massimo, Ciccaglione, Anna Rita, Conti, Fabrizio, Ceccarelli, Fulvia, Lo Presti, Alessandra
Format: Article
Language:English
Subjects:
autoantibodies
Autoimmune diseases
Biotechnology
Case Report
Datasets
Deoxyribonucleic acid
DNA
Genetic aspects
Genotype & phenotype
Hepatitis B
Hepatitis B virus
Infections
Liver cancer
Lupus
Lupus erythematosus
Mutation
mutations
phylogenetic analysis
Phylogenetics
Rheumatology
Viral infections
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hepatitis B virus (HBV) infection is a serious global health problem. Patients with autoimmune diseases, such as Lupus Erythematosus, are exposed to a higher risk of acquiring infections. In this study, a molecular characterization, genomic investigation of the Hepatitis B virus, polymerase (P) and surface (S) genes, from a patient affected by Cutaneous Lupus Erythematosus (CLE), was presented. Viral DNA was extracted from 200 μL of serum, and the HBV-DNA was amplified by real-time polymerase chain reaction (PCR) with the Platinum Taq DNA Polymerase. The PCR products were purified and sequencing reactions were performed. A phylogenetic analysis was performed through maximum likelihood and Bayesian approaches. The HBV CLE isolate was classified as sub-genotype D3 and related to other Italian HBV D3 genomes, and some from foreign countries. No drug resistant mutations were identified. One mutation (a.a. 168 M) was located in the last part of the major hydrophilic region (MHR) of the surface antigen (HBsAg). Moreover, three sites (351G, 526Y, 578C) in the polymerase were exclusively present in the CLE patient. The mutations identified exclusively in the HBsAg of our CLE patient may have been selected because of the Lupus autoantibodies, which are characteristic in the Lupus autoimmune disease, using a possible molecular mimicry mechanism.
ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics12112866