A porcine model to study the effect of brain death on kidney genomic responses

A majority of transplanted organs come from donors after brain death (BD). Renal grafts from these donors have higher delayed graft function and lower long-term survival rates compared to living donors. We designed a novel porcine BD model to better delineate the incompletely understood inflammatory...

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Bibliographic Details
Published in:Journal of clinical and translational science 2018-08, Vol.2 (4), p.208-216
Main Authors: Sally, Mitchell B, Malinoski, Darren J, Zaldivar, Frank P, Le, Tony, Khoshnevis, Matin, Pinette, William A, Hutchens, Michael, Radom-Aizik, Shlomit
Format: Article
Language:English
Subjects:
Antigens
Balloon catheters
Basic and Preclinical Research
Basic translational research
Biopsy
Blood & organ donations
Brain death
Brain research
Cell adhesion & migration
Cell adhesion molecules
Cold storage
Critical care
Cytokines
donor management
Gene expression
genomic
Inflammation
Ischemia
Kidney transplantation
Kidneys
Nephrology
Organ donors
Physiology
porcine model
Ribonucleic acid
RNA
Rodents
transplantation
Transplants & implants
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Summary:A majority of transplanted organs come from donors after brain death (BD). Renal grafts from these donors have higher delayed graft function and lower long-term survival rates compared to living donors. We designed a novel porcine BD model to better delineate the incompletely understood inflammatory response to BD, hypothesizing that adhesion molecule pathways would be upregulated in BD. Animals were anesthetized and instrumented with monitors and a balloon catheter, then randomized to control and BD groups. BD was induced by inflating the balloon catheter and animals were maintained for 6 hours. RNA was extracted from kidneys, and gene expression pattern was determined. In total, 902 gene pairs were differently expressed between groups. Eleven selected pathways were upregulated after BD, including cell adhesion molecules. These results should be confirmed in human organ donors. Treatment strategies should target involved pathways and lessen the negative effects of BD on transplantable organs.
ISSN:2059-8661
2059-8661
DOI:10.1017/cts.2018.312