Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic development

Bacteria of the genus Streptomyces have a linear chromosome, with a core region and two ‘arms’. During their complex life cycle, these bacteria develop multi-genomic hyphae that differentiate into chains of exospores that carry a single copy of the genome. Sporulation-associated cell division requir...

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Bibliographic Details
Published in:Nature communications 2021-09, Vol.12 (1), p.5222-5222, Article 5222
Main Authors: Szafran, Marcin J., Małecki, Tomasz, Strzałka, Agnieszka, Pawlikiewicz, Katarzyna, Duława, Julia, Zarek, Anna, Kois-Ostrowska, Agnieszka, Findlay, Kim C., Le, Tung B. K., Jakimowicz, Dagmara
Format: Article
Language:English
Subjects:
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Bacteria
Cell division
Chromosomes
Condensin
Eukaryotes
Genomics
Humanities and Social Sciences
Hyphae
Life cycles
multidisciplinary
Proteins
Science
Science (multidisciplinary)
Sporulation
Streptomyces
Streptomyces venezuelae
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Summary:Bacteria of the genus Streptomyces have a linear chromosome, with a core region and two ‘arms’. During their complex life cycle, these bacteria develop multi-genomic hyphae that differentiate into chains of exospores that carry a single copy of the genome. Sporulation-associated cell division requires chromosome segregation and compaction. Here, we show that the arms of Streptomyces venezuelae chromosomes are spatially separated at entry to sporulation, but during sporogenic cell division they are closely aligned with the core region. Arm proximity is imposed by segregation protein ParB and condensin SMC. Moreover, the chromosomal terminal regions are organized into distinct domains by the Streptomyces -specific HU-family protein HupS. Thus, as seen in eukaryotes, there is substantial chromosomal remodelling during the Streptomyces life cycle, with the chromosome undergoing rearrangements from an ‘open’ to a ‘closed’ conformation. Streptomyces bacteria have a linear chromosome and a complex life cycle, including development of multi-genomic hyphae that differentiate into mono-genomic exospores. Here, Szafran et al. show that the chromosome of Streptomyces venezuelae undergoes substantial remodelling during sporulation, from an ‘open’ to a ‘closed’ conformation.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-021-25461-2