TRPV1 Supports Axogenic Enhanced Excitability in Response to Neurodegenerative Stress

• Published in: Frontiers in cellular neuroscience 2021-01, Vol.14, p.603419
• Main Authors: Risner, Michael L, McGrady, Nolan R, Boal, Andrew M, Pasini, Silvia, Calkins, David J
• Format: Article
• Language: English
• Subjects: axon; Axons; Blindness; Capsaicin receptors; Cellular Neuroscience; dendritic pruning; Depolarization; Disease; Excitability; Excitatory postsynaptic potentials; Experiments; Glaucoma; Influence; Laboratories; Light; Neurodegeneration; Neurodegenerative diseases; Optic nerve; Physiology; Retina; Retinal ganglion cells; Sodium channels (voltage-gated); Transient receptor potential proteins; TRPV1
• ISSN: 1662-5102; 1662-5102
• DOI: 10.3389/fncel.2020.603419

Early progression in neurodegenerative disease involves challenges to homeostatic processes, including those controlling axonal excitability and dendritic organization. In glaucoma, the leading cause of irreversible blindness, stress from intraocular pressure (IOP) causes degeneration of retinal ganglion cells (RGC) and their axons which comprise the optic nerve. Previously, we discovered that early progression induces axogenic, voltage-gated enhanced excitability of RGCs, even as dendritic complexity in the retina reduces. Here, we investigate a possible contribution of the transient receptor potential vanilloid type 1 (TRPV1) channel to enhanced excitability, given its role in modulating excitation in other neural systems. We find that genetic deletion of ( ) influences excitability differently for RGCs firing continuously to light onset (αON-Sustained) vs. light offset (αOFF-Sustained). Deletion drives excitability in opposing directions so that RGC responses with elevated IOP equalize to that of wild-type (WT) RGCs without elevated IOP. Depolarizing current injections in the absence of light-driven presynaptic excitation to directly modulate voltage-gated channels mirrored these changes, while inhibiting voltage-gated sodium channels and isolating retinal excitatory postsynaptic currents abolished both the differences in light-driven activity between WT and RGCs and changes in response due to IOP elevation. Together, these results support a voltage-dependent, axogenic influence of with elevated IOP. Finally, slowed the loss of dendritic complexity with elevated IOP, opposite its effect on axon degeneration, supporting the idea that axonal and dendritic degeneration follows distinctive programs even at the level of membrane excitability.