Analysis of endophytic bacterial diversity in seeds of different genotypes of cotton and the suppression of Verticillium wilt pathogen infection by a synthetic microbial community

• Published in: BMC plant biology 2024-04, Vol.24 (1), p.263-263, Article 263
• Main Authors: Wu, Chong-Die, Fan, Yong-Bin, Chen, Xue, Cao, Jiang-Wei, Ye, Jing-Yi, Feng, Meng-Lei, Liu, Xing-Xing, Sun, Wen-Jing, Liu, Rui-Na, Wang, Ai-Ying
• Format: Article
• Language: English
• Subjects: Agricultural production; Agriculture; Analysis; Bacteria; Bacteria - genetics; Biological diversity; Community structure; Cotton; Cotton industry; Cotton Verticillium wilt; Disease Resistance - genetics; Ecological distribution; Ecological effects; Ecological niches; Endophytes; Environmental aspects; Fertilizers; Fungal diseases; Gene amplification; Gene sequencing; Genetic aspects; Genetic testing; Genotype; Genotype & phenotype; Genotypes; Gossypium; Gossypium - genetics; Gossypium - microbiology; Health aspects; High-throughput sequencing; Information storage; Medical research; Medicine, Experimental; Microbial activity; Microbial community construction; Microbiological strains; Microbiomes; Microbiota; Microorganisms; Niche (Ecology); Niches; Pathogens; Plant diseases; Plant Diseases - microbiology; Plant tissues; Ribosomal RNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Seed microbiome; Seeds; Seeds - genetics; Strains (organisms); Symbiosis; Synthetic Microbial Community (SynCom); Verticillium; Verticillium - physiology; Verticillium wilt
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-04910-2

In agricultural production, fungal diseases significantly impact the yield and quality of cotton (Gossypium spp.) with Verticillium wilt posing a particularly severe threat. This study is focused on investigating the effectiveness of endophytic microbial communities present in the seeds of disease-resistant cotton genotypes in the control of cotton Verticillium wilt. The technique of 16S ribosomal RNA (16S rRNA) amplicon sequencing identified a significant enrichment of the Bacillus genus in the resistant genotype Xinluzao 78, which differed from the endophytic bacterial community structure in the susceptible genotype Xinluzao 63. Specific enriched strains were isolated and screened from the seeds of Xinluzao 78 to further explore the biological functions of seed endophytes. A synthetic microbial community (SynCom) was constructed using the broken-rod model, and seeds of the susceptible genotype Xinluzao 63 in this community that had been soaked with the SynCom were found to significantly control the occurrence of Verticillium wilt and regulate the growth of cotton plants. Antibiotic screening techniques were used to preliminarily identify the colonization of strains in the community. These techniques revealed that the strains can colonize plant tissues and occupy ecological niches in cotton tissues through a priority effect, which prevents infection by pathogens. This study highlights the key role of seed endophytes in driving plant disease defense and provides a theoretical basis for the future application of SynComs in agriculture.