Assessment of dengue virus inactivation in random donor platelets using amotosalen and ultraviolet A illumination

OBJECTIVES: The study objective was evaluation of amotosalen and ultraviolet A (UVA) illumination-based inactivation of dengue virus (DENV) in blood platelets. MATERIALS AND METHODS: Whole blood was collected from healthy donors and platelet concentrates were prepared at a tertiary care hospital in...

Full description

Saved in:
Bibliographic Details
Published in:Asian journal of transfusion science 2022-01, Vol.16 (1), p.67-72
Main Authors: Kumar, Ankit, Tiwari, Aseem, Kumar, Satendra, Biswas, Ashutosh, Singh, Gurpreet, Chatterjee, Kabita, Chakroborty, Sourit, Sunil, Sujatha
Format: Article
Language:English
Subjects:
amotosalen
Analysis
Blood platelets
Chemical compounds
Clinical outcomes
Dengue fever
dengue virus inactivation
Dengue viruses
Original
Photochemistry
photoinactivation
random donor platelets
transfusion
transfusion transmitted infection
Ultraviolet radiation
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:OBJECTIVES: The study objective was evaluation of amotosalen and ultraviolet A (UVA) illumination-based inactivation of dengue virus (DENV) in blood platelets. MATERIALS AND METHODS: Whole blood was collected from healthy donors and platelet concentrates were prepared at a tertiary care hospital in Gurugram, India. Platelet units collected from five blood group matched individuals were pooled and spiked with DENV. The spiked platelet units were subjected to amotosalen treatment followed by UVA illumination, to evaluate the efficiency of this method for viral inactivation. The treated platelet units were evaluated for the presence of infectious DENV. Amotosalen levels were quantified in the treated samples using high-performance liquid chromatography. RESULTS: The presence of replicating DENV was not observed in spiked platelet units treated with amotosalen and UVA illumination, whereas untreated units contained actively replicating DENV. Amotosalen levels were found to be in the permissible range after photochemical inactivation. CONCLUSIONS: Amotosalen/UVA pathogen inactivation treatment showed efficient inactivation of DENV in platelet components. Therefore, it seems to be a promising method for mitigating the risk of dengue transmission through transfusion of potentially contaminated platelet components in dengue-endemic countries such as India.
ISSN:0973-6247
1998-3565
DOI:10.4103/ajts.AJTS_108_19