Role of heat shock protein 47 in transdifferentiation of human tenon's fibroblasts to myofibroblasts

Heat shock protein 47 (Hsp47) is a well-known molecular chaperone in collagen synthesis and maturation. The aim of this study is to investigate its putative role in the transdifferentiation of Tenon's fibroblasts to myofibroblasts. Primary cultured human Tenon's fibroblasts were exposed to...

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Published in:BMC ophthalmology 2012-09, Vol.12 (1), p.49-49, Article 49
Main Authors: Hong, Samin, Park, Kyoungsoo, Kim, Jin Hyoung, Han, Sueng-Han, Lee, Jong Bok, Seong, Gong Je
Format: Article
Language:English
Subjects:
Actin
Analysis
Biotechnology
Blotting, Western
Bone morphogenetic proteins
Cell culture
Cell Transdifferentiation - physiology
Cells, Cultured
Collagen
Fibroblast
Fibroblasts
Fibroblasts - cytology
Fibroblasts - metabolism
Fibrosis
Fluorides
Gene Expression Regulation
Heat shock protein
Heat shock proteins
HSP47 Heat-Shock Proteins - physiology
Humans
Muscle proteins
Myofibroblast
Myofibroblasts - cytology
Myofibroblasts - metabolism
Ophthalmology
Physiological aspects
Real time
Real-Time Polymerase Chain Reaction
RNA
RNA, Messenger - genetics
Smooth muscle
Transdifferentiation
Transforming growth factor-β
Transforming growth factors
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Summary:Heat shock protein 47 (Hsp47) is a well-known molecular chaperone in collagen synthesis and maturation. The aim of this study is to investigate its putative role in the transdifferentiation of Tenon's fibroblasts to myofibroblasts. Primary cultured human Tenon's fibroblasts were exposed to transforming growth factor-β1 (TGF-β1) for up to 48 hours. The mRNA levels of Hsp47 and α smooth muscle actin (αSMA) were determined by quantitative real time RT-PCR. After delivery of small interfering RNA (siRNA) molecules targeting Hsp47 into the cells, the expression of Hsp47 and αSMA proteins was determined by western immunoblotting. TGF-β1 increased the mRNA expressions of both Hsp47 and αSMA in human Tenon's fibroblasts, as determined by quantitative real time RT-PCR. However, it induced the protein expression of only αSMA but not Hsp47, as determined by western immunoblots. When siRNAs specific for Hsp47 were introduced into those cells, the TGF-β1-induced expression of αSMA was significantly attenuated on western immunoblots; after 48 hours of exposure to TGF-β1, the relative densities of immunobands were 11.58 for the TGF-β1 only group and 2.75 for the siRNA treatment group, compared with the no treatment control group (p 
ISSN:1471-2415
1471-2415
DOI:10.1186/1471-2415-12-49