Risk of wild fungi treatment failure: Phallus impudicus-induced telomere damage triggers p21/p53 and p16-dependent cell cycle arrest and may contribute to male fertility reduction in vitro

• Published in: Ecotoxicology and environmental safety 2021-02, Vol.209, p.111782, Article 111782
• Main Authors: Solek, Przemyslaw, Shemedyuk, Nataliya, Shemedyuk, Anastasiya, Dudzinska, Ewa, Koziorowski, Marek
• Format: Article
• Language: English
• Subjects: Agaricales - metabolism; Apoptosis; Basidiomycota; Cell Cycle; Cell Cycle Checkpoints - drug effects; DNA Damage; Fertility - drug effects; GC-1 spg & GC-2 spd; Humans; Male; Men fertility reduction; Mycotoxins - toxicity; Phallus impudicus; Telomere; Telomere damage; Treatment Failure; Tumor Suppressor Protein p53 - metabolism
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2020.111782

The multifunctional characteristics of Phallus impudicus extract encourage to conduct research for its potential use in medical applications. Well, science is constantly seeking new evidence for the biological activity of extracts of natural origin. Drugs of natural origin should not cause any side effects on the physiological functions of the human body; however, this is not always successful. In this study, we used in vitro approach to evaluate the toxicity of alcohol Phallus impudicus extract on spermatogenic cells. We show, for the first time, cytotoxic properties of Phallus impudicus treatment associated with a decrease in cellular metabolic activity, dysregulation of redox homeostasis and impairment of selected antioxidant cell protection systems. As a consequence, p53/p21- and p16-mediated cell cycle arrest followed by p27 activation is initiated. The observed changes were associated with telomere shortening and numerous DNA damage at the chromosome ends (altered expression pattern of TRF1 and TRF2 proteins), as well as upregulation of cleaved caspase-3 with a decrease in Bcl-2 expression, suggesting induction of apoptotic death. Therefore, these results provide molecular evidence for mechanistic pathways and novel adverse outcomes linked to the Phallus impudicus treatment towards men's health and fertility reduction. •Phallus impudicus extract induces toxic events in spermatogenic cells.•Phallus impudicus-induced dysregulation of redox homeostasis.•Phallus impudicus-induced p53/p21- and p16-mediated cell cycle arrest.•Phallus impudicus treatment contributes to men’s health and fertility reduction.