PASTMUS: mapping functional elements at single amino acid resolution in human cells

Identification of functional elements for a protein of interest is important for achieving a mechanistic understanding. However, it remains cumbersome to assess each and every amino acid of a given protein in relevance to its functional significance. Here, we report a strategy, PArsing fragmented DN...

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Bibliographic Details
Published in:Genome Biology 2019-12, Vol.20 (1), p.279-16, Article 279
Main Authors: Zhang, Xinyi, Yue, Di, Wang, Yinan, Zhou, Yuexin, Liu, Ying, Qiu, Yeting, Tian, Feng, Yu, Ying, Zhou, Zhuo, Wei, Wensheng
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Bioinformatics
Cell Cycle Proteins - chemistry
CRISPR
Deoxyribonucleic acid
DNA
Genes
Genomes
Genotype & phenotype
Heparin-binding EGF-like Growth Factor - chemistry
Humans
Method
Mutagenesis
Mutation
Nucleotide sequence
Polo-Like Kinase 1
Protein Serine-Threonine Kinases - chemistry
Proteins
Proto-Oncogene Proteins - chemistry
Sequence Analysis, Protein - methods
Structure-Activity Relationship
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Summary:Identification of functional elements for a protein of interest is important for achieving a mechanistic understanding. However, it remains cumbersome to assess each and every amino acid of a given protein in relevance to its functional significance. Here, we report a strategy, PArsing fragmented DNA Sequences from CRISPR Tiling MUtagenesis Screening (PASTMUS), which provides a streamlined workflow and a bioinformatics pipeline to identify critical amino acids of proteins in their native biological contexts. Using this approach, we map six proteins-three bacterial toxin receptors and three cancer drug targets, and acquire their corresponding functional maps at amino acid resolution.
ISSN:1474-760X
1474-7596
1474-760X
DOI:10.1186/s13059-019-1897-7