Arsenic Trioxide Triggers Apoptosis of Metastatic Oral Squamous Cells Carcinoma with Concomitant Downregulation of GLI1 in Hedgehog Signaling

Given the lack of advances in Oral Squamous Cell Carcinoma (OSCC) therapy in recent years, pharmacological strategies to block OSCC-related signaling pathways have gained prominence. The present study aimed to evaluate the therapeutic potential of Arsenic Trioxide (ATO) concerning its antitumoral ef...

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Published in:Biomedicines 2022-12, Vol.10 (12), p.3293
Main Authors: Nogueira, Raphael Luís Rocha, de Araújo, Taís Bacelar Sacramento, Valverde, Ludmila Faro, Silva, Viviane Aline Oliveira, Cavalcante, Bruno Raphael Ribeiro, Rossi, Erik Aranha, Allahdadi, Kyan James, Dos Reis, Mitermayer Galvão, Pereira, Thiago Almeida, Coletta, Ricardo D, Bezerra, Daniel Pereira, de Freitas Souza, Bruno Solano, Dias, Rosane Borges, Rocha, Clarissa A Gurgel
Format: Article
Language:English
Subjects:
Angiogenesis
Apoptosis
Arsenic
Arsenic compounds
Arsenic trioxide
Cancer
Cell cycle
Cell death
Cell morphology
Cell viability
Cytology
Cytotoxicity
DNA fragmentation
drug repurposing
Experiments
Fibroblasts
GLI1
Health aspects
Hedgehog protein
hedgehog signaling
Medical prognosis
Metastases
Metastasis
Morphology
Mouth cancer
oral cancer
Oral carcinoma
Oral squamous cell carcinoma
Physiological aspects
Signal transduction
Transcription factors
Tumors
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Summary:Given the lack of advances in Oral Squamous Cell Carcinoma (OSCC) therapy in recent years, pharmacological strategies to block OSCC-related signaling pathways have gained prominence. The present study aimed to evaluate the therapeutic potential of Arsenic Trioxide (ATO) concerning its antitumoral effects and the inhibition of the Hedgehog (HH) pathway in OSCC. Initially, ATO cytotoxicity was assessed in a panel of cell lines. Cell viability, cell cycle, death patterns, and cell morphology were analyzed, as well as the effect of ATO on the expression of HH pathway components. After the cytotoxic assay, HSC3 cells were chosen for all in vitro assays. ATO increased apoptotic cell death and nuclear fragmentation in the sub-G1 cell cycle phase and promoted changes in cell morphology. In addition, the reduced expression of GLI1 indicated that ATO inhibits HH activity. The present study provides evidence of ATO as an effective cytotoxic drug for oral cancer treatment in vitro.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines10123293